Specifications
- Purity
- ≥99% (Titration)
- Appearance
- White powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (6M), ethanol (1M)
- Melting Point
- 178-185 °C
- Density
- 1.345 g/cm3 at 20 °C
Available from stock
Guanidine hydrochloride
- SKU
- G0040
Categories:
- Synonyms
- Aminoformamidine hydrochloride , Aminomethanamidine hydrochloride , Guanidinium chloride , Carbamimidoylazanium chloride
- 50-01-1
- CAS-Number
- CH5N3 . HCl
- Molecular Formula
- 59.07 . 36.46
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Hazard statements
- H302 + H332-H315-H319
- Precautionary statements
- P261-P280-P301 + P312 + P330-P304 + P340 + P312-P305 + P351 + P338-P337 + P313
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Guanidine hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. It is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies and to increase the solubility of hydrophobic molecules. When utilized at lower concentrations, it is useful in prompting the refolding of denatured proteins. Guanidine hydrochloride is also been reported to allow return of enzymatic activity in protocols using it as a first step. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
- Smiles
- NC([NH3+])=N.[Cl-]
- InChi Key
- PJJJBBJSCAKJQF-UHFFFAOYSA-N
- References
- (1) C. Holm, et al., Gene 42, 169 (1986) , (2) C.N. Pace, et al., Biochemistry 29, 2564 (1990) , (3) F.A. Marston, et al., Meth. Enzymol. 182, 264 (1990) , (4) R. Rudolph & H. Lilie, FASEB J. 10, 49 (1996) , (5) A. Mukhopadhyay, Adv. Biochem. Eng. Biotechnol. 56, 61 (1997) , (6) S. Emadi & M. Behzadi, BBRC 450, 1339 (2014)
- InChi
- InChI=1S/CH5N3.ClH/c2-1(3)4 , /h(H5,2,3,4) , 1H
Guanidine hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. It is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies and to increase the solubility of hydrophobic molecules. When utilized at lower concentrations, it is useful in prompting the refolding of denatured proteins. Guanidine hydrochloride is also been reported to allow return of enzymatic activity in protocols using it as a first step. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Additional information
| Synonyms | Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride, Carbamimidoylazanium chloride |
|---|---|
| Purity | ≥99% (Titration) |
| Appearance | White powder |
| CAS-Number | 50-01-1 |
| Molecular Formula | CH5N3 . HCl |
| Molecular Weight | 59.07 . 36.46 |
| Identity | 1H-NMR |
| Solvents | water (6M), ethanol (1M) |
| Melting Point | 178-185 °C |
| Density | 1.345 g/cm3 at 20 °C |
| Smiles | NC([NH3+])=N.[Cl-] |
| InChi Key | PJJJBBJSCAKJQF-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Hazard statements | H302 + H332-H315-H319 |
| Precautionary statements | P261-P280-P301 + P312 + P330-P304 + P340 + P312-P305 + P351 + P338-P337 + P313 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) C. Holm, et al., Gene 42, 169 (1986), (2) C.N. Pace, et al., Biochemistry 29, 2564 (1990), (3) F.A. Marston, et al., Meth. Enzymol. 182, 264 (1990), (4) R. Rudolph & H. Lilie, FASEB J. 10, 49 (1996), (5) A. Mukhopadhyay, Adv. Biochem. Eng. Biotechnol. 56, 61 (1997), (6) S. Emadi & M. Behzadi, BBRC 450, 1339 (2014) |
| InChi | InChI=1S/CH5N3.ClH/c2-1(3)4, /h(H5,2,3,4), 1H |
| Quantity | 100 g, 500 g, Bulk |
