Specifications
- Purity
- ≥96% (TLC)
- Appearance
- Yellow, dark yellow to beige powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO (10mg/ml) or methanol (1mg/ml).
- Melting Point
- 237 °C (dec.)(lit.)
- Fluorescence
- λex 355 nm, λem ~430 nm in methanol
Category: Fluorescent Detection, Fluorescent Detection
TMA-DPH is a fluorescent probe for membrane fluidity measurements in artificial and living membrane systems. Acts as an anchor at the lipid/water interface. Specifically and rapidly incorporating into the plasma membrane and between the membranes and the buffer. It is especially useful in the study of monolayer dynamics of lipoproteins and similar analogous systems, exocytosis kinetics and similar systems. TMA-DPH is cylinder-shaped and has fluorescence emission transition dipoles that are basically aligned parallel to its long molecular axis. As such, it is very sensitive to reorientation resulting from interactions with surrounding lipids. TMA-DPH is widely used in fluorescence polarization studies of rotational motion. Spectral Data: λex 355 nm, λem ~430 nm in methanol or DMSO.
Synonyms | N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate, 1,6-Diphenyl-1,3,5-hexatriene-4'-trimethylammonium tosylate, 1-(4-Trimethylammoniophenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate |
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Purity | ≥96% (TLC) |
Appearance | Yellow, dark yellow to beige powder |
CAS-Number | 115534-33-3 |
Molecular Formula | C21H24N · C7H7O3S |
Molecular Weight | 461.62 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO (10mg/ml) or methanol (1mg/ml). |
Melting Point | 237 °C (dec.)(lit.) |
Fluorescence | λex 355 nm, λem ~430 nm in methanol |
Smiles | C[N+](C)(C)C(C=C1)=CC=C1/C=C/C=C/C=C/C2=CC=CC=C2.CC3=CC=C(S([O-])(=O)=O)C=C3 |
InChi Key | ZKARERKEBVSZCX-VMDDUYISSA-M |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) D. Illinger, et al., Cell Biophys. 14, 17 (1989), (2) C. Maire, et al., Biorheology 29, 507 (1992), (3) G. Ferretti, et al., Biochim. Biophys. Acta 1147, 245 (1993), (4) D. Illinger, et al., J. Cell Biol. 125, 783 (1994), (5) D. Illinger, et al., Biochim. Biophys. Acta 1239, 58 (1995), (6) J. Matko & P. Nagy, J. Photochem. Photobiol. B 40, 120 (1997), (7) C. Saldana, et al., J. Membr. Biol. 190, 75 (2002), (8) B. Chazotte, Cold Spring Harb. Protoc. (2011), (9) A.M.T.M. do Canto, et al., Biochim. Biophys. Acta 1858, 2647 (2016) |
Quantity | 25 mg, Bulk |