Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Yellow to brown powder
- Identity
- 1H-NMR
Properties
- Solvents
- ethanol, DMSO, DMF
Category: Fluorescent Detection, Fluorescent Detection
6-Fluorescein isothiocyanate (6-FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Spectral data: ?ex=492nm, ?em=518nm.
Synonyms | Fluorescein 6-isothiocyanate, Fluorescein-4-isothiocyanate, 2-(3-Hydroxy-6-oxoxanthen-9-yl)-4-isothiocyanatobenzoic acid, FITC Isomer II, FITC-II |
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Purity | ≥90% (HPLC) |
Appearance | Yellow to brown powder |
CAS-Number | 18861-78-4 |
Molecular Formula | C21H11NO5S |
Molecular Weight | 389.39 |
Identity | 1H-NMR |
Solvents | ethanol, DMSO, DMF |
Smiles | O=C(C1=C2C=C(N=C=S)C=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53 |
InChi Key | GTQFZXYECNSNNC-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H302, H315, H317, H319, H332, H334 , H335 |
Precautionary statements | P261, P264, P270-P272, P280, P285, P363, P301+P312, P302+P352, P304+P340, P305+P351+P338, P342+P311, P403+P233, P405, P501 |
GHS Symbol | GHS07+GHS08 |
Signal word | Danger |
Transportation | Not dangerous goods |
References | (1) T.J. Porro, et al., Stain Technol. 38, 37 (1963), (2) H.S. Corey & R.M. McKinney, Anal. Biochem. 10, 387 (1965), (3) R.M. McKinney, et al., Anal. Biochem. 29, 526 (1969), (4) J. Sinsheimer, et al., Anal. Biochem. 57, 227 (1974), (5) D.L. Reynolds & L.A. Pachla, J. Pharm. Sci. 74, 1091 (1985), (6) R. Zuckermann, et al., Nucl. Acids Res. 15, 5305 (1987), (7) C.A. Swindlehurst & E.W. Voss, Biophys. J. 59, 619 (1991), (8) C. Wang, et al., BBRC 331, 351 (2005) |
Quantity | 100 mg, 250 mg, Bulk |