Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow to yellow-green crystalline solid
- Identity
- 1H-NMR
Properties
- Solvents
- water (50 mg/ml), DMSO
- Fluorescence
- λex 355 nm, λem 465 nm in TE buffer
Downloads
- Safety Data Sheet
- CDX B0030 MSDS.pdf
Category: Fluorescent Detection, Fluorescent Detection
Name | Hoechst 33342 | Hoechst 33258 | Hoechst 34580 | Hoechst S769121 (Nuclear Yellow) |
SKU | B0030 | B0029 | B0094 | H0328 |
CAS | 875756-97-1 | 23491-45-4 | 911004-45-0 | 74681-68-8 |
Ex/Em (nm) | 352/454 | 352/454 | 371/438 | 372/504 |
Molecular Weight | 561.93 | 533.88 | 560.95 | 596.96 |
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells.
Other available Hoechst dyes:
Name | Hoechst 33342 | Hoechst 33258 | Hoechst 34580 | Hoechst S769121 (Nuclear Yellow) |
SKU | B0030 | B0029 | B0094 | H0328 |
CAS | 875756-97-1 | 23491-45-4 | 911004-45-0 | 74681-68-8 |
Ex/Em (nm) | 352/454 | 352/454 | 371/438 | 372/504 |
Molecular Weight | 561.93 | 533.88 | 560.95 | 596.96 |
Synonyms | 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride |
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Purity | ≥98% (HPLC) |
Appearance | Yellow to yellow-green crystalline solid |
CAS-Number | 23491-52-3 |
Molecular Formula | C27H28N6O . 3HCl . xH2O |
Molecular Weight | 561.93 |
Identity | 1H-NMR |
Solvents | water (50 mg/ml), DMSO |
Fluorescence | λex 355 nm, λem 465 nm in TE buffer |
Smiles | CCOC1=CC=C(C=C1)C1=NC2=C(N1)C=C(C=C2)C1=NC2=CC(=CC=C2N1)N1CCN(C)CC1 |
InChi Key | PRDFBSVERLRRMY-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H302-H315-H335-H341 |
Precautionary statements | P280-P301 + P312 + P330 |
GHS Symbol | GHS07+GHS08 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) D.J. Pierce et al.: Exp. Hematol. 35(9), 1437 (2007), (2) M.E. Lalande et al.: Proc. Natl. Acad. Sci. USA 78, 363 (1981), (3) M.J. Lydon et al.: J. Cell. Physiol. 102, 175 (1980) |
InChi | InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31) |
Quantity | 25 mg, 500 mg, 5 g, Bulk |