Available from stock

Hoechst 33342

SKU: B0030

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride; 23491-52-3
CAS-Number: C₂₇H₂₈N₆O . ₃HCl . xH₂O
Molecular Formula: 561.93
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: Yellow to yellow-green crystalline solid
Identity: 1H-NMR

Properties

Solvents: water (50 mg/ml), DMSO
Fluorescence: λex 355 nm, λem 465 nm in TE buffer

Documentation

Safety Data Sheet (SDS): CDX B0030 MSDS_V1.2.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H302-H315-H335-H341
Precautionary statements: P280-P301 + P312 + P330
GHS Symbol: GHS07+GHS08
Signal word: Warning
Transportation: Not dangerous goods

Description

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells.

Other available Hoechst dyes:

Name	Hoechst 33342	Hoechst 33258	Hoechst 34580	Hoechst S769121 (Nuclear Yellow)
SKU	B0030	B0029	B0094	H0328
CAS	875756-97-1	23491-45-4	911004-45-0	74681-68-8
Ex/Em (nm)	352/454	352/454	371/438	372/504
Molecular Weight	561.93	533.88	560.95	596.96

Smiles

CCOC1=CC=C(C=C1)C1=NC2=C(N1)C=C(C=C2)C1=NC2=CC(=CC=C2N1)N1CCN(C)CC1

InChi Key

PRDFBSVERLRRMY-UHFFFAOYSA-N

References

(1) D.J. Pierce et al.: Exp. Hematol. 35(9), 1437 (2007) , (2) M.E. Lalande et al.: Proc. Natl. Acad. Sci. USA 78, 363 (1981) , (3) M.J. Lydon et al.: J. Cell. Physiol. 102, 175 (1980)

InChi

InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)

Other available Hoechst dyes:

Name	Hoechst 33342	Hoechst 33258	Hoechst 34580	Hoechst S769121 (Nuclear Yellow)
SKU	B0030	B0029	B0094	H0328
CAS	875756-97-1	23491-45-4	911004-45-0	74681-68-8
Ex/Em (nm)	352/454	352/454	371/438	372/504
Molecular Weight	561.93	533.88	560.95	596.96

SKU: B0030 Categories: Fluorescent Detection, Fluorescent Detection

Description
Additional information

Description

Additional information

Synonyms	2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity	≥98% (HPLC)
Appearance	Yellow to yellow-green crystalline solid
CAS-Number	23491-52-3
Molecular Formula	C27H28N6O . 3HCl . xH2O
Molecular Weight	561.93
Identity	1H-NMR
Solvents	water (50 mg/ml), DMSO
Fluorescence	λex 355 nm, λem 465 nm in TE buffer
Smiles	CCOC1=CC=C(C=C1)C1=NC2=C(N1)C=C(C=C2)C1=NC2=CC(=CC=C2N1)N1CCN(C)CC1
InChi Key	PRDFBSVERLRRMY-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302-H315-H335-H341
Precautionary statements	P280-P301 + P312 + P330
GHS Symbol	GHS07+GHS08
Signal word	Warning
Transportation	Not dangerous goods
References	(1) D.J. Pierce et al.: Exp. Hematol. 35(9), 1437 (2007), (2) M.E. Lalande et al.: Proc. Natl. Acad. Sci. USA 78, 363 (1981), (3) M.J. Lydon et al.: J. Cell. Physiol. 102, 175 (1980)
InChi	InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)
Quantity	25 mg, 500 mg, 5 g, Bulk