Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Light yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0
Downloads
- Safety Data Sheet
- CDX D0084 MSDS.pdf
Category: Fluorescent Detection, Fluorescent Detection
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell’s cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
Synonyms | 4,5-Diaminofluorescein, 5,6-Diaminofluorescein |
---|---|
Purity | ≥95% (HPLC) |
Appearance | Light yellow powder |
CAS-Number | 205391-01-1 |
Molecular Formula | C20H14N2O5 |
Molecular Weight | 362.34 |
Identity | 1H-NMR |
Solvents | DMSO |
Fluorescence | λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0 |
Smiles | NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
InChi Key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H302 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) M. Kirsch et al.: J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al.: J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.: Anal. Chem. 70, 2446 (1998) |
InChi | InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 |
Quantity | 1 mg, 5 mg, 10 mg, Bulk |