5-Aminofluorescein

Specifications

Purity

≥95% (HPLC), 6-Isomer ≤1%

Appearance

Beige to brown powder

Identity

1H-NMR

Properties

UV / Vis

λmax 496 nm in borate buffer pH 8.5

Solvents

acetone, methanol, DMSO

Handling Advice

Protect from light and moisture.

Use / Stability

Stable for at least 2 years after receipt when stored at +4°C.

Long Term Storage

+4°C

Short Term Storage

+4°C

Shipping

AMBIENT

Transportation

Not dangerous goods

Signal word

Warning

GHS Symbol

GHS07

Precautionary statements

P261, P305 + P351 + P338

Hazard statements

H315, H319, H335

Description

The uronic acid residues of all known glycosaminoglycuronans react wit 5-aminofluorescein to yield fluorescent derivatives, which show fluorescence characteristics identical to those of 5-acetamidofluorescein (CDX-A0089). 5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020).

InChi

InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2

References

(1) O.N. Burchak et al.: Bioorg. & Med. Chem. 14(8), 2559 (2006) , (2) H. Sigmund et al.: Helv. Chim. Acta, 86(7), 2299 (2003) , (3) A. Ogamo et al.: Carboh. Res. 105(1), 69 (1982)

InChi Key

GZAJOEGTZDUSKS-UHFFFAOYSA-N

Smiles

NC1=CC=C2C(=C1)C(=O)OC21C2=CC=C(O)C=C2OC2=CC(O)=CC=C12