Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Powder
Properties
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
- Solvents
- DMSO or DMF
Available from stock
5-CFDA N-succinimidyl ester
- SKU
- C0094
Category: Fluorescent Detection
- Synonyms
- 5-Carboxyfluorescein diacetate N-succinimidyl ester
- 150206-05-6
- CAS-Number
- C29H19NO11
- Molecular Formula
- 557.46
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- 5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
- InChi
- InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
- References
- (1) G.O. Bakhus. Et al.: Immunologiya 23(3), 190 (2002) , (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000) , (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (4) S. Boitano et al.: J. Cell Sci. 98, 343 (1991)
- InChi Key
- SRSXVRUMXPCNAJ-UHFFFAOYSA-N
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
Additional information
| Synonyms | 5-Carboxyfluorescein diacetate N-succinimidyl ester |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Powder |
| CAS-Number | 150206-05-6 |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.46 |
| Identity | 1H-NMR |
| Solvents | DMSO or DMF |
| Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) G.O. Bakhus. Et al.: Immunologiya 23(3), 190 (2002), (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000), (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996), (4) S. Boitano et al.: J. Cell Sci. 98, 343 (1991) |
| InChi | InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3 |
| Quantity | 5 mg, 25 mg, 100 mg, Bulk |
