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    5-CFDA N-succinimidyl ester

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    5-CFDA N-succinimidyl ester

    SKU: C0094

    Category: Fluorescent Detection

    • Product Data
    • Handling
    • Details
    • Synonyms: 5-Carboxyfluorescein diacetate N-succinimidyl ester
    • Purity: ≥95% (HPLC)
    • Appearance: Powder.
    • CAS-Number: 150206-05-6
    • Molecular Formula: C29H19NO11
    • Molecular Weight: 557.46
    • Identity: 1H-NMR
    • Solvents: DMSO or DMF
    • Fluorescence: λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
    • Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
    • InChi: InChI=1S/C12H9NO3/c1-2-15-10-4-3-8-5-9(7-13)12(14)16-11(8)6-10/h3-6H,2H2,1H3
    • InChi Key: SRSXVRUMXPCNAJ-UHFFFAOYSA-N
    • Shipping: AMBIENT
    • Short Term Storage: +4°C
    • Long Term Storage: -20°C
    • Handling Advice: Protect from light and moisture.
    • Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
    • Description: 5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
    • References: (1) M.T. Donato et al.; Drug Metabol. Disp. 32(7), 699 (2004), (2) C.L. Crespi et al.; Anal. Biochem. 248(1), 188 (1997), (3) I.N.H. White et al.; Anal. Biochem. 172(2), 304 (1988)

    5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.

    SKU: C0094 Category: Fluorescent Detection
    • Additional information
    • Reviews (0)

    Additional information

    Identity

    1H-NMR

    Handling Advice

    Protect from light and moisture.

    Availability

    1

    CAS-Number

    150206-05-6

    Long Term Storage

    -20°C

    InChi Key

    SRSXVRUMXPCNAJ-UHFFFAOYSA-N

    Quantity

    100 mg, 25 mg, 5 mg, Bulk

    Appearance

    Powder.

    InChi

    InChI=1S/C12H9NO3/c1-2-15-10-4-3-8-5-9(7-13)12(14)16-11(8)6-10/h3-6H,2H2,1H3

    Fluorescence

    λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

    Molecular Formula

    C29H19NO11

    Short Term Storage

    +4°C

    Purity

    ≥95% (HPLC)

    Pricetable

    100 mg_EUR_890, 25 mg_EUR_290, 5 mg_EUR_115

    References

    (1) M.T. Donato et al.; Drug Metabol. Disp. 32(7), 699 (2004), (2) C.L. Crespi et al.; Anal. Biochem. 248(1), 188 (1997), (3) I.N.H. White et al.; Anal. Biochem. 172(2), 304 (1988)

    Smiles

    CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2

    Use / Stability

    Stable for at least 2 years after receipt when stored at -20°C.

    Molecular Weight

    557.46

    Solvents

    DMSO or DMF

    Synonyms

    5-Carboxyfluorescein diacetate N-succinimidyl ester

    Shipping

    AMBIENT

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