5-CFDA N-succinimidyl ester

SKU: C0094

Category: Fluorescent Detection

Synonyms: 5-Carboxyfluorescein diacetate N-succinimidyl ester; 150206-05-6
CAS-Number: C₂₉H₁₉NO₁₁
Molecular Formula: 557.46
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Identity: 1H-NMR
Appearance: Powder

Properties

Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Solvents: DMSO or DMF

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: 5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
InChi: InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
References: (1) G.O. Bakhus. Et al.: Immunologiya 23(3), 190 (2002) , (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000) , (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (4) S. Boitano et al.: J. Cell Sci. 98, 343 (1991)
InChi Key: SRSXVRUMXPCNAJ-UHFFFAOYSA-N
Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2

5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.

SKU: C0094 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5-Carboxyfluorescein diacetate N-succinimidyl ester
Purity	≥95% (HPLC)
Appearance	Powder
CAS-Number	150206-05-6
Molecular Formula	C29H19NO11
Molecular Weight	557.46
Identity	1H-NMR
Solvents	DMSO or DMF
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Smiles	CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key	SRSXVRUMXPCNAJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) G.O. Bakhus. Et al.: Immunologiya 23(3), 190 (2002), (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000), (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996), (4) S. Boitano et al.: J. Cell Sci. 98, 343 (1991)
InChi	InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
Quantity	5 mg, 25 mg, 100 mg, Bulk