- Synonyms: 5-Carboxyfluorescein diacetate N-succinimidyl ester
- Purity: ≥95% (HPLC)
- Appearance: Powder.
- CAS-Number: 150206-05-6
- Molecular Formula: C29H19NO11
- Molecular Weight: 557.46
- Identity: 1H-NMR
- Solvents: DMSO or DMF
- Fluorescence: λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
- Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
- InChi: InChI=1S/C12H9NO3/c1-2-15-10-4-3-8-5-9(7-13)12(14)16-11(8)6-10/h3-6H,2H2,1H3
- InChi Key: SRSXVRUMXPCNAJ-UHFFFAOYSA-N
Available from stock
5-CFDA N-succinimidyl ester
SKU: C0094
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: 5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
- References: (1) M.T. Donato et al.; Drug Metabol. Disp. 32(7), 699 (2004), (2) C.L. Crespi et al.; Anal. Biochem. 248(1), 188 (1997), (3) I.N.H. White et al.; Anal. Biochem. 172(2), 304 (1988)
5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 150206-05-6 |
| Long Term Storage | -20°C |
| InChi Key | SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
| Quantity | 100 mg, 25 mg, 5 mg, Bulk |
| Appearance | Powder. |
| InChi | InChI=1S/C12H9NO3/c1-2-15-10-4-3-8-5-9(7-13)12(14)16-11(8)6-10/h3-6H,2H2,1H3 |
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) M.T. Donato et al.; Drug Metabol. Disp. 32(7), 699 (2004), (2) C.L. Crespi et al.; Anal. Biochem. 248(1), 188 (1997), (3) I.N.H. White et al.; Anal. Biochem. 172(2), 304 (1988) |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO or DMF |
| Synonyms | 5-Carboxyfluorescein diacetate N-succinimidyl ester |
| Shipping | AMBIENT |
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