Available from stock

5-CFDA

SKU: C0039

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 5-Carboxyfluorescein diacetate; 79955-27-4
CAS-Number: C₂₅H₁₆O₉
Molecular Formula: 460.39
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Appearance: White to yellow solid
Identity: 1H-NMR

Properties

Solvents: DMSO, chloroform
Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

Documentation

Safety Data Sheet (SDS): CDX C0039 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Transportation: Not dangerous goods

Description: Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.
Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key: WPUZGNPQMIWOHE-UHFFFAOYSA-N
References: (1) J. Immun. Methods 172, 115 (1994) , (2) Anticancer Res. 14, 927 (1994) , (3) Appl. Environ Microbiol. 60, 1467 (1994) , (4) Cytometry 15, 213 (1994) , (5) Biol. Reprod. 34, 127 (1986) , (6) Immun. Lett. 2, 187 (1981)
InChi: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.

SKU: C0039 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	5-Carboxyfluorescein diacetate
Purity	≥95% (HPLC)
Appearance	White to yellow solid
CAS-Number	79955-27-4
Molecular Formula	C25H16O9
Molecular Weight	460.39
Identity	1H-NMR
Solvents	DMSO, chloroform
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Smiles	CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key	WPUZGNPQMIWOHE-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) J. Immun. Methods 172, 115 (1994), (2) Anticancer Res. 14, 927 (1994), (3) Appl. Environ Microbiol. 60, 1467 (1994), (4) Cytometry 15, 213 (1994), (5) Biol. Reprod. 34, 127 (1986), (6) Immun. Lett. 2, 187 (1981)
InChi	InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
Quantity	25 mg, 100 mg, 1 g, Bulk