Specifications
- Purity
- TLC-Test
- Appearance
- Dark violet powder
Properties
- Solvents
- DMF, acetonitrile, DMSO
- Fluorescence
- λex 575 nm, λem 600 nm in 0.1 M phosphate pH 7.0
Available from stock
5-ROX N-succinimidyl ester
- SKU
- C0019
Category: Fluorescent Detection
- Synonyms
- 5-Carboxy-X-rhodamine succinimidyl ester, 5-ROX SE, 5-CXR SE
- 209734-74-7
- CAS-Number
- C37H33N3O7
- Molecular Formula
- 631.67
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P264-P271-P280-P302+P352-P305+P351+P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- 5-ROX N-succinimidyl ester (5-ROX-SE) is an amine-reactive long wavelength rhodamine dye. Derivatives made with the amine-reactive carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red® conjugates. 5-ROX-SE derivatized compounds such as proteins, nucleic acids and drugs may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. 5-ROX-SE is a labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing. 5-ROX-SE is a single isomer of ROX and increasingly preferred for labeling peptides and nucleotides because it gives better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. Spectral data: λex=578nm, λem=604nm, Extinction coefficient (cm-1M-1): 82000, Quantum yield: 0.94 (aqueous buffer pH 7.0).
- Smiles
- [O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O
- InChi Key
- BTTBJYLMDASSAS-UHFFFAOYSA-N
- References
- (1) L.G. Lee, et al., Nucl. Acids Res. 20, 2471 (1992) , (2) J. Ju, et al., PNAS 92, 4347 (1995) , (3) S.-C. Hung, et al., Anal. Biochem. 255, 32 (1998) , (4) Y. Yoshikawa, et al., Anal. Biochem. 256, 82 (1998) , (5) P.J. Finn, et al., Nucl. Acids Res. 30, 2877 (2002) , (6) T.S. Seo, et al., PNAS 102, 5926 (2005)
- InChi
- InChi=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2
5-ROX N-succinimidyl ester (5-ROX-SE) is an amine-reactive long wavelength rhodamine dye. Derivatives made with the amine-reactive carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red® conjugates. 5-ROX-SE derivatized compounds such as proteins, nucleic acids and drugs may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. 5-ROX-SE is a labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. 5-ROX-SE is a single isomer of ROX and increasingly preferred for labeling peptides and nucleotides because it gives better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. Spectral data: λex=578nm, λem=604nm, Extinction coefficient (cm-1M-1): 82000, Quantum yield: 0.94 (aqueous buffer pH 7.0).
Additional information
| Synonyms | 5-Carboxy-X-rhodamine succinimidyl ester, 5-ROX SE, 5-CXR SE |
|---|---|
| Purity | TLC-Test |
| Appearance | Dark violet powder |
| CAS-Number | 209734-74-7 |
| Molecular Formula | C37H33N3O7 |
| Molecular Weight | 631.67 |
| Solvents | DMF, acetonitrile, DMSO |
| Fluorescence | λex 575 nm, λem 600 nm in 0.1 M phosphate pH 7.0 |
| Smiles | [O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O |
| InChi Key | BTTBJYLMDASSAS-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P264-P271-P280-P302+P352-P305+P351+P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) L.G. Lee, et al., Nucl. Acids Res. 20, 2471 (1992), (2) J. Ju, et al., PNAS 92, 4347 (1995), (3) S.-C. Hung, et al., Anal. Biochem. 255, 32 (1998), (4) Y. Yoshikawa, et al., Anal. Biochem. 256, 82 (1998), (5) P.J. Finn, et al., Nucl. Acids Res. 30, 2877 (2002), (6) T.S. Seo, et al., PNAS 102, 5926 (2005) |
| InChi | InChi=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2 |
| Quantity | 5 mg, Bulk |
| cdx-Emission max. | 595 – 608 nm |
