Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Off-white to light yellow solid
- Identity
- 1H-NMR
Properties
- Solvents
- water, DMSO
- Fluorescence
- λex 355 nm, la.em 495 nm in 0.1 M Tris pH 8.0, Ca2+
Available from stock
Fura-2, pentapotassium salt
- SKU
- F0013
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Fura-2 . 5K
- 113694-64-7
- CAS-Number
- C29H22N3O14 . 5K
- Molecular Formula
- 831.99
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Very light-sensitive. Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: λex 363nm, λem 512nm in water.
- Smiles
- [O-]C(C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)=O.[K+].[K+].[K+].[K+].[K+]
- InChi Key
- HQKWBBCTCLUGSX-UHFFFAOYSA-I
- References
- (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (2) D. Brethes, et al., PNAS 84, 1439 (1987) , (3) W.F. Goldman, et al., Cell Calcium. 11, 221 (1990) , (4) M.W. Roe, et al., Cell Calcium. 11, 63 (1990) , (5) S.K. Kong & C.Y. Lee, Biochem. Mol. Biol. Ed. 23, 97 (1995) , (6) C. Cheng & I.J. Reynolds, J. Neurochem. 71, 2401 (1998) , (7) B.M. Palmer & R.L. Moore, Am. J. Physiol. Cell Physiol. 2000, C1278 (2000) , (8) J.L. Martin, et al., Cell Calcium 40, 393 (2006)
- InChi
- InChI=1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40,,,,,/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42),,,,,/q,5*+1/p-5
Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: λex 363nm, λem 512nm in water.
Additional information
| Synonyms | Fura-2 . 5K |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | Off-white to light yellow solid |
| CAS-Number | 113694-64-7 |
| Molecular Formula | C29H22N3O14 . 5K |
| Molecular Weight | 831.99 |
| Identity | 1H-NMR |
| Solvents | water, DMSO |
| Fluorescence | λex 355 nm, la.em 495 nm in 0.1 M Tris pH 8.0, Ca2+ |
| Smiles | [O-]C(C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)=O.[K+].[K+].[K+].[K+].[K+] |
| InChi Key | HQKWBBCTCLUGSX-UHFFFAOYSA-I |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Very light-sensitive. Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (2) D. Brethes, et al., PNAS 84, 1439 (1987), (3) W.F. Goldman, et al., Cell Calcium. 11, 221 (1990), (4) M.W. Roe, et al., Cell Calcium. 11, 63 (1990), (5) S.K. Kong & C.Y. Lee, Biochem. Mol. Biol. Ed. 23, 97 (1995), (6) C. Cheng & I.J. Reynolds, J. Neurochem. 71, 2401 (1998), (7) B.M. Palmer & R.L. Moore, Am. J. Physiol. Cell Physiol. 2000, C1278 (2000), (8) J.L. Martin, et al., Cell Calcium 40, 393 (2006) |
| InChi | InChI=1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40,,,,,/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42),,,,,/q,5*+1/p-5 |
| Quantity | 1 mg, Bulk |
