Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Deep blue to deep violet powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, methanol
- Fluorescence
- λex 512 nm, λem 567 nm (acid/neutral)λex 598 nm, λem 668 nm (basic)
- Other
- Emission ≤ 672 ± 5 nm
Category: Fluorescent Detection
5(6)-Carboxynaphthofluorescein (CNF) is a fluorescent NIR emitting pH indicator with red fluorescence at pH 9 (Spectral: Ex/Em: 598/668 nm). The fluorescence shifs to shorter wavelengths (Ex/Em: 512/567nm) at neutral or acidic pH environments. Spectral data: λex=512nm, λem=567nm (acid/neutral), λex=598nm, λem=668nm (basic). CNF shows good sensitivity in an alkaline pH range and it can be exploited in the construction of fiber-optic pH sensors. CNF has been used as an intravesicular pH probe for the colorimetric detection of activity, selectivity and cooperativity of ion channels. CNF has been used to design a luminescent probe for determination of pH, based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and CNF as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.
Synonyms | CNF |
---|---|
Purity | ≥90% (HPLC) |
Appearance | Deep blue to deep violet powder |
CAS-Number | 128724-35-6 |
Molecular Formula | C29H16O7 |
Molecular Weight | 476.43 |
Identity | 1H-NMR |
Solvents | DMSO, methanol |
Fluorescence | λex 512 nm, λem 567 nm (acid/neutral)λex 598 nm, λem 668 nm (basic) |
Other | Emission ≤ 672 ± 5 nm |
Smiles | OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc5cc(O)ccc5c4Oc6c3ccc7cc(O)ccc67.OC(=O)c8ccc9C(=O)OC(cccccc(O)ccccOccccccc(O)ccc)c9c8 |
InChi Key | ANUHNEJUELPITC-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
Transportation | Not dangerous goods |
References | (1) A. Song, et al., Anal. Chem. 69, 863 (1997), (2) S.M. Butterfield, et al., Org. Biomol. Chem. 7, 1784 (2009), (3) R.J. Meier, et al., Chem. Commun. 51, 6145 (2015), (4) E. Poehler, et al., Anal. Bioanal. Chem. 408, 2927 (2016), (5) D.A. Jankowska, et al., Biosens. Bioelectr. 87, 312 (2017), (6) S. Nevolova, et al., Biotech. J. 14, 1800144 (2019) |
InChi | InChi=1S/2C29H16O7/c30-17-4-6-19-14(11-17)1-9-23-25(19)35-26-20-7-5-18(31)12-15(20)2-10-24(26)29(23)22-8-3-16(27(32)33)13-21(22)28(34)36-29,30-17-4-7-19-14(11-17)2-9-22-25(19)35-26-20-8-5-18(31)12-15(20)3-10-23(26)29(22)24-13-16(27(32)33)1-6-21(24)28(34)36-29/h2*1-13,30-31H,(H,32,33) |
Quantity | 25 mg, 100 mg, Bulk |