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      5(6)-TAMRA N-succinimidyl ester

      SKU
      C0027

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      5(6)-TAMRA SE , 5(6)-Carboxytetramethylrhodamine NHS , 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester , 246256-50-8
      150408-83-6
      CAS-Number
      C29H25N3O7
      Molecular Formula
      527.52
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥90% (HPLC)
      Appearance
      Dark brown powder
      Identity
      1H-NMR

      Properties

      Solvents
      DMF, acetonitrile, methanol, DMSO

      Documentation

      Safety Data Sheet (SDS)
      CDX C0027 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Transportation
      Not dangerous goods
      Description
      Amine-reactive form of 5(6)-carboxytetramethylrhodamine. Conjugates yield bright, pH-insensitive orange-red fluorescence (Ex/Em: ~543/576nm i MeOH) with good photostability. Fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates or other primary amine-containing molecules. Especially also used for fluorescent antibodies and avidin derivatives used in immunochemistry.
      Smiles
      CN(C)C1=CC=C(C(C2=CC=CC=C2C([O-])=O)=C(C=C/3)C(O4)=CC3=[N+](C)C)C4=C1.O=C(C)ON5C(CCC5=O)=O
      InChi Key
      MCDQUPOEOXETSJ-UHFFFAOYSA-N
      References
      (1) E. Koller Appl. Fluoresc. Technol. 3, 20, (1991) , (2) L.D. Mayfield, D.R. Corey Bioorg. Med. Chem. Lett. 9, 14, (1999) , (3) M. Hahn, et al., Electrophoresis 22, 2691 (2001) , (4) T.M. Hsu, et al., Clin. Chem. 47, 1373 (2001) , (5) C. Olivier, et al., Bioconjug. Chem 14, 430 (2003) , (6) H.Y. Li, et al., J. Cell Biol. 160, 635 (2003) , (7) W. Erker, et al., BBRC 324, 893 (2004)
      InChi
      InChI=1S/C24H22N2O3.C6H7NO4/c1-25(2)15-9-11-19-21(13-15)29-22-14-16(26(3)4)10-12-20(22)23(19)17-7-5-6-8-18(17)24(27)28,1-4(8)11-7-5(9)2-3-6(7)10/h5-14H,1-4H3,2-3H2,1H3

      Amine-reactive form of 5(6)-carboxytetramethylrhodamine. Conjugates yield bright, pH-insensitive orange-red fluorescence (Ex/Em: ~543/576nm i MeOH) with good photostability. Fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates or other primary amine-containing molecules. Especially also used for fluorescent antibodies and avidin derivatives used in immunochemistry.

      SKU: C0027 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      5(6)-TAMRA SE, 5(6)-Carboxytetramethylrhodamine NHS, 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester, 246256-50-8

      Purity

      ≥90% (HPLC)

      Appearance

      Dark brown powder

      CAS-Number

      150408-83-6

      Molecular Formula

      C29H25N3O7

      Molecular Weight

      527.52

      Identity

      1H-NMR

      Solvents

      DMF, acetonitrile, methanol, DMSO

      Smiles

      CN(C)C1=CC=C(C(C2=CC=CC=C2C([O-])=O)=C(C=C/3)C(O4)=CC3=[N+](C)C)C4=C1.O=C(C)ON5C(CCC5=O)=O

      InChi Key

      MCDQUPOEOXETSJ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) E. Koller Appl. Fluoresc. Technol. 3, 20, (1991), (2) L.D. Mayfield, D.R. Corey Bioorg. Med. Chem. Lett. 9, 14, (1999), (3) M. Hahn, et al., Electrophoresis 22, 2691 (2001), (4) T.M. Hsu, et al., Clin. Chem. 47, 1373 (2001), (5) C. Olivier, et al., Bioconjug. Chem 14, 430 (2003), (6) H.Y. Li, et al., J. Cell Biol. 160, 635 (2003), (7) W. Erker, et al., BBRC 324, 893 (2004)

      InChi

      InChI=1S/C24H22N2O3.C6H7NO4/c1-25(2)15-9-11-19-21(13-15)29-22-14-16(26(3)4)10-12-20(22)23(19)17-7-5-6-8-18(17)24(27)28,1-4(8)11-7-5(9)2-3-6(7)10/h5-14H,1-4H3,2-3H2,1H3

      Quantity

      25 mg, Bulk

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