5(6)-TAMRA N-succinimidyl ester

SKU: C0027

Category: Fluorescent Detection

Synonyms: 5(6)-TAMRA SE , 5(6)-Carboxytetramethylrhodamine NHS , 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester , 246256-50-8; 150408-83-6
CAS-Number: C₂₉H₂₅N₃O₇
Molecular Formula: 527.52
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Identity: 1H-NMR
Appearance: Dark brown powder

Properties

Solvents: DMF, acetonitrile, methanol, DMSO

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: Amine-reactive form of 5(6)-carboxytetramethylrhodamine. Conjugates yield bright, pH-insensitive orange-red fluorescence (Ex/Em: ~543/576nm i MeOH) with good photostability. Fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates or other primary amine-containing molecules. Especially also used for fluorescent antibodies and avidin derivatives used in immunochemistry.
InChi: InChI=1S/C24H22N2O3.C6H7NO4/c1-25(2)15-9-11-19-21(13-15)29-22-14-16(26(3)4)10-12-20(22)23(19)17-7-5-6-8-18(17)24(27)28,1-4(8)11-7-5(9)2-3-6(7)10/h5-14H,1-4H3,2-3H2,1H3
References: (1) E. Koller Appl. Fluoresc. Technol. 3, 20, (1991) , (2) L.D. Mayfield, D.R. Corey Bioorg. Med. Chem. Lett. 9, 14, (1999) , (3) M. Hahn, et al., Electrophoresis 22, 2691 (2001) , (4) T.M. Hsu, et al., Clin. Chem. 47, 1373 (2001) , (5) C. Olivier, et al., Bioconjug. Chem 14, 430 (2003) , (6) H.Y. Li, et al., J. Cell Biol. 160, 635 (2003) , (7) W. Erker, et al., BBRC 324, 893 (2004)
InChi Key: MCDQUPOEOXETSJ-UHFFFAOYSA-N
Smiles: CN(C)C1=CC=C(C(C2=CC=CC=C2C([O-])=O)=C(C=C/3)C(O4)=CC3=[N+](C)C)C4=C1.O=C(C)ON5C(CCC5=O)=O

Amine-reactive form of 5(6)-carboxytetramethylrhodamine. Conjugates yield bright, pH-insensitive orange-red fluorescence (Ex/Em: ~543/576nm i MeOH) with good photostability. Fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates or other primary amine-containing molecules. Especially also used for fluorescent antibodies and avidin derivatives used in immunochemistry.

SKU: C0027 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5(6)-TAMRA SE, 5(6)-Carboxytetramethylrhodamine NHS, 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester, 246256-50-8
Purity	≥90% (HPLC)
Appearance	Dark brown powder
CAS-Number	150408-83-6
Molecular Formula	C29H25N3O7
Molecular Weight	527.52
Identity	1H-NMR
Solvents	DMF, acetonitrile, methanol, DMSO
Smiles	CN(C)C1=CC=C(C(C2=CC=CC=C2C([O-])=O)=C(C=C/3)C(O4)=CC3=[N+](C)C)C4=C1.O=C(C)ON5C(CCC5=O)=O
InChi Key	MCDQUPOEOXETSJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) E. Koller Appl. Fluoresc. Technol. 3, 20, (1991), (2) L.D. Mayfield, D.R. Corey Bioorg. Med. Chem. Lett. 9, 14, (1999), (3) M. Hahn, et al., Electrophoresis 22, 2691 (2001), (4) T.M. Hsu, et al., Clin. Chem. 47, 1373 (2001), (5) C. Olivier, et al., Bioconjug. Chem 14, 430 (2003), (6) H.Y. Li, et al., J. Cell Biol. 160, 635 (2003), (7) W. Erker, et al., BBRC 324, 893 (2004)
InChi	InChI=1S/C24H22N2O3.C6H7NO4/c1-25(2)15-9-11-19-21(13-15)29-22-14-16(26(3)4)10-12-20(22)23(19)17-7-5-6-8-18(17)24(27)28,1-4(8)11-7-5(9)2-3-6(7)10/h5-14H,1-4H3,2-3H2,1H3
Quantity	25 mg, Bulk