Specifications
- Purity
- ≥99% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- water
Documentation
- Safety Data Sheet (SDS)
- CDX A0306 MSDS.pdf
Available from stock
6-Amino-D-luciferin
- SKU
- A0306
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4,5-dihydro-2[6-amino-2-benzthiazolyl]-4-thiazole carboxylic acid
- 161055-47-6
- CAS-Number
- C11H9N3O2S2
- Molecular Formula
- 279.34
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Description
- Amino analog of the common D-luciferin substrate for use in measuring firefly luciferase activity. This cell permeable reagent can be used when considering design of proluminescent bioconjugates (peptidase substrates) of luciferin for ultrasensitive luminescent assays. In bioluminescent protease assays peptides or amino acids are conjugated to the luciferin analog 6-amino-D-luciferin. These substrates are useful for ultrasensitive analysis of a variety of protease enzymes including various caspases and cathepsins, chymotrypsin, trypsin, elastase, kallikrein, thrombin, ficin, bromoalain, plasmin, papain, ficin and many others or even for use in vivo tracing of luciferase transfected cells in living tissues. The resulting bioluminescence spectrum of reaction of 6-Amino-D-luciferin is very similar to that observed for the natural substrate D-luciferin. Peptidase assays using a bioluminogenic substrate can be easily adapted to 96-well formats for HTS-based assays. Using this principle, it is possible to design an assay to detect almost any peptidase or protease activity via bioluminescence by using a substrate with a suitable peptide sequence. Applications in pathogen detection, discovery of protease inhibitors, probing cell physiology and assessing protease activity in oncogenesis are possible at extraordinary sensitivity.
- Smiles
- C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)N)C(=O)O
- InChi Key
- HKSJKXOOBAVPKR-SSDOTTSWSA-N
- References
- (1) R. Shinde, et al., Biochemistry 45, 11103 (2006) , (2) A. Dragulescu-Andrasi, et al., Bioconjugate Chem. 20, 1660 (2009) , (3) H. Takakura, et al., Chem. Asian J. 5, 2053 (2010) , (4) K.R. Harwood, et al., Chem. Biol. 18, 1649 (2011) , (5) S.J. Orcutt, et al., BBA Mol. Res. 1823, 2079 (2012) , (6) Z-S. Li, et al., J. Photochem. Photobiol. A: Chem. 243, 7 (2012) , (7) R. Kojima, et al., Angew. Chem. Int. Ed. 52, 1175 (2013) , (8) J. Li, et al., Anal. Chem. 86, 2747 (2014) , (9) V.R. Viviani, et al., Biochemistry 53, 5208 (2014) , (10) D.M. Mofford, et al., JACS 136, 13277 (2014) , (11) Y. Nam, et al., Chem. Commun. 52, 1128 (2016)
Amino analog of the common D-luciferin substrate for use in measuring firefly luciferase activity. This cell permeable reagent can be used when considering design of proluminescent bioconjugates (peptidase substrates) of luciferin for ultrasensitive luminescent assays. In bioluminescent protease assays peptides or amino acids are conjugated to the luciferin analog 6-amino-D-luciferin. These substrates are useful for ultrasensitive analysis of a variety of protease enzymes including various caspases and cathepsins, chymotrypsin, trypsin, elastase, kallikrein, thrombin, ficin, bromoalain, plasmin, papain, ficin and many others or even for use in vivo tracing of luciferase transfected cells in living tissues. The resulting bioluminescence spectrum of reaction of 6-Amino-D-luciferin is very similar to that observed for the natural substrate D-luciferin. Peptidase assays using a bioluminogenic substrate can be easily adapted to 96-well formats for HTS-based assays. Using this principle, it is possible to design an assay to detect almost any peptidase or protease activity via bioluminescence by using a substrate with a suitable peptide sequence. Applications in pathogen detection, discovery of protease inhibitors, probing cell physiology and assessing protease activity in oncogenesis are possible at extraordinary sensitivity.
Additional information
| Synonyms | 4,5-dihydro-2[6-amino-2-benzthiazolyl]-4-thiazole carboxylic acid |
|---|---|
| Purity | ≥99% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 161055-47-6 |
| Molecular Formula | C11H9N3O2S2 |
| Molecular Weight | 279.34 |
| Identity | 1H-NMR |
| Solvents | water |
| Smiles | C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)N)C(=O)O |
| InChi Key | HKSJKXOOBAVPKR-SSDOTTSWSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| References | (1) R. Shinde, et al., Biochemistry 45, 11103 (2006), (2) A. Dragulescu-Andrasi, et al., Bioconjugate Chem. 20, 1660 (2009), (3) H. Takakura, et al., Chem. Asian J. 5, 2053 (2010), (4) K.R. Harwood, et al., Chem. Biol. 18, 1649 (2011), (5) S.J. Orcutt, et al., BBA Mol. Res. 1823, 2079 (2012), (6) Z-S. Li, et al., J. Photochem. Photobiol. A: Chem. 243, 7 (2012), (7) R. Kojima, et al., Angew. Chem. Int. Ed. 52, 1175 (2013), (8) J. Li, et al., Anal. Chem. 86, 2747 (2014), (9) V.R. Viviani, et al., Biochemistry 53, 5208 (2014), (10) D.M. Mofford, et al., JACS 136, 13277 (2014), (11) Y. Nam, et al., Chem. Commun. 52, 1128 (2016) |
| Quantity | 1 mg, 5 mg, Bulk |
