Available from stock

6-CFDA N-succinimidyl ester

SKU: C0095

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 6-Carboxyfluorescein diacetate N-succinimidyl ester; 150206-15-8
CAS-Number: C₂₉H₁₉NO₁₁
Molecular Formula: 557.46
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Appearance: Light yellow powder
Identity: 1H-NMR

Properties

Solvents: DMSO or DMF
Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

Documentation

Safety Data Sheet (SDS): CDX C0095 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: 6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein. Reagent for continuously determining the intracellular pH in bacteria by fluorometry. Labeling human intervertebral disk cells in vitro for fluorescence microscopy.
Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key: JGPOSNWWINVNFV-UHFFFAOYSA-N
References: (1) G.O. Bakhus. et al.: B.V. Pinegin: Immunologiya 23(3), 190 (2002) , (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000) , (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (4) C.S. Chen et al.: JBC, 268(21), 15812 (1993)
InChi: InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein. Reagent for continuously determining the intracellular pH in bacteria by fluorometry. Labeling human intervertebral disk cells in vitro for fluorescence microscopy.

SKU: C0095 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	6-Carboxyfluorescein diacetate N-succinimidyl ester
Purity	≥90% (HPLC)
Appearance	Light yellow powder
CAS-Number	150206-15-8
Molecular Formula	C29H19NO11
Molecular Weight	557.46
Identity	1H-NMR
Solvents	DMSO or DMF
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Smiles	CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key	JGPOSNWWINVNFV-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) G.O. Bakhus. et al.: B.V. Pinegin: Immunologiya 23(3), 190 (2002), (2) H.E. Gruber et al.: Biotech. Histochem. 75, 118 (2000), (3) P. Breeuwer et al.: Appl. Environ. Microbiol. 62, 178 (1996), (4) C.S. Chen et al.: JBC, 268(21), 15812 (1993)
InChi	InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
Quantity	5 mg, 25 mg, 100 mg, Bulk