6-CFDA

SKU: C0040

Category: Fluorescent Detection

Synonyms: 6-Carboxyfluorescein diacetate; 3348-03-6
CAS-Number: C₂₅H₁₆O₉
Molecular Formula: 460.39
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Identity: 1H-NMR
Appearance: White to yellow powder

Properties

Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Solvents: DMSO

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
InChi: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
References: (1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998) , (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997) , (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994)
InChi Key: QMOGCCYGOPYYNT-UHFFFAOYSA-N
Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2

Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.

SKU: C0040 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	6-Carboxyfluorescein diacetate
Purity	≥95% (HPLC)
Appearance	White to yellow powder
CAS-Number	3348-03-6
Molecular Formula	C25H16O9
Molecular Weight	460.39
Identity	1H-NMR
Solvents	DMSO
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Smiles	CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
InChi Key	QMOGCCYGOPYYNT-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998), (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997), (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994)
InChi	InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
Quantity	25 mg, 100 mg, 1 g, Bulk