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    6-CFDA

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      6-CFDA

      SKU
      C0040

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      6-Carboxyfluorescein diacetate
      3348-03-6
      CAS-Number
      C25H16O9
      Molecular Formula
      460.39
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (HPLC)
      Appearance
      White to yellow powder
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO
      Fluorescence
      λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

      Documentation

      Safety Data Sheet (SDS)
      CDX C0040 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      +4°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at +4°C.
      Transportation
      Not dangerous goods
      Description
      Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
      Smiles
      CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
      InChi Key
      QMOGCCYGOPYYNT-UHFFFAOYSA-N
      References
      (1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998) , (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997) , (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994)
      InChi
      InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

      Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.

      SKU: C0040 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      6-Carboxyfluorescein diacetate

      Purity

      ≥95% (HPLC)

      Appearance

      White to yellow powder

      CAS-Number

      3348-03-6

      Molecular Formula

      C25H16O9

      Molecular Weight

      460.39

      Identity

      1H-NMR

      Solvents

      DMSO

      Fluorescence

      λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

      Smiles

      CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2

      InChi Key

      QMOGCCYGOPYYNT-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      +4°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at +4°C.

      Transportation

      Not dangerous goods

      References

      (1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998), (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997), (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994)

      InChi

      InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

      Quantity

      25 mg, 100 mg, 1 g, Bulk

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