Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- White to yellow powder
Properties
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
- Solvents
- DMSO
Available from stock
6-CFDA
- SKU
- C0040
Category: Fluorescent Detection
- Synonyms
- 6-Carboxyfluorescein diacetate
- 3348-03-6
- CAS-Number
- C25H16O9
- Molecular Formula
- 460.39
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
- InChi
- InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
- References
- (1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998) , (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997) , (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994)
- InChi Key
- QMOGCCYGOPYYNT-UHFFFAOYSA-N
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
Additional information
| Synonyms | 6-Carboxyfluorescein diacetate |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | White to yellow powder |
| CAS-Number | 3348-03-6 |
| Molecular Formula | C25H16O9 |
| Molecular Weight | 460.39 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | QMOGCCYGOPYYNT-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) C. Dive et al.: Mol. Cell. Probes 2, 131 (1998), (2) N. Yamaguchi et al.: J. Appl. Microbiol. 83, 43 (1997), (3) P.J. Oefner et al.: Anal. Biochem. 223, 39 (1994) |
| InChi | InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
| Quantity | 25 mg, 100 mg, 1 g, Bulk |
