- Synonyms: 6-Carboxyfluorescein diacetate
- Purity: ≥95% (HPLC)
- Appearance: White to yellow powder.
- CAS-Number: 3348-03-6
- Molecular Formula: C25H16O9
- Molecular Weight: 460.39
- Identity: 1H-NMR
- Solvents: DMSO
- Fluorescence: λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
- Smiles: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
- InChi: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
- InChi Key: QMOGCCYGOPYYNT-UHFFFAOYSA-N
Available from stock
6-CFDA
SKU: C0040
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: +4°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
- Description: Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
- References: (1) J. Immun. Methods 172, 115 (1994), (2) Anticancer Res. 14, 927 (1994), (3) Appl. Environ Microbiol. 60, 1467 (1994), (4) Cytometry 15, 213 (1994), (5) Biol. Reprod. 34, 127 (1986), (6) Immun. Lett. 2, 187 (1981)
Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enter cells and are hydrolysed more slowly than FDA. CFDA and FDA hydrolyses obey Michaelis-Menten kinetics with Km values of around 19 and 2 microM, respectively.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 3348-03-6 |
| Long Term Storage | +4°C |
| InChi Key | QMOGCCYGOPYYNT-UHFFFAOYSA-N |
| Quantity | 1 g, 100 mg, 25 mg, Bulk |
| Appearance | White to yellow powder. |
| InChi | InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) J. Immun. Methods 172, 115 (1994), (2) Anticancer Res. 14, 927 (1994), (3) Appl. Environ Microbiol. 60, 1467 (1994), (4) Cytometry 15, 213 (1994), (5) Biol. Reprod. 34, 127 (1986), (6) Immun. Lett. 2, 187 (1981) |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Molecular Weight | |
| Solvents | DMSO |
| Synonyms | 6-Carboxyfluorescein diacetate |
| Shipping | AMBIENT |
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