Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Yellow solid
Properties
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
- Melting Point
- >300 °C
- Solvents
- DMSO, water (pH ≥ 5)
Downloads
- Safety Data Sheet
- CDX C0016 MSDS.pdf
Categories: Fluorescent Detection, Fluorescent Detection
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
Synonyms | 6-Carboxyfluorescein |
---|---|
Purity | ≥98% (HPLC) |
Appearance | Yellow solid |
CAS-Number | 3301-79-9 |
Molecular Formula | C21H12O7 |
Molecular Weight | 376.32 |
Identity | 1H-NMR |
Solvents | DMSO, water (pH ≥ 5) |
Melting Point | >300 °C |
Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 |
Smiles | OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
InChi Key | YILMHDCPZJTMGI-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H315, H319, H335 |
Precautionary statements | P261, P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) Bioconjugate Chem. 6, 447 (1995) |
InChi | InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27) |
Quantity | 50 mg, 200 mg, 1 g, Bulk |