6-FAM

SKU: C0016

Category: Fluorescent Detection

Synonyms: 6-Carboxyfluorescein; 3301-79-9
CAS-Number: C₂₁H₁₂O₇
Molecular Formula: 376.32
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Yellow solid

Properties

Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
Melting Point: >300 °C
Solvents: DMSO, water (pH ≥ 5)

Long Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P261, P305 + P351 + P338
Hazard statements: H315, H319, H335

Description: The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
InChi: InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27)
References: (1) Bioconjugate Chem. 6, 447 (1995)
InChi Key: YILMHDCPZJTMGI-UHFFFAOYSA-N
Smiles: OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12

The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.

SKU: C0016 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	6-Carboxyfluorescein
Purity	≥98% (HPLC)
Appearance	Yellow solid
CAS-Number	3301-79-9
Molecular Formula	C21H12O7
Molecular Weight	376.32
Identity	1H-NMR
Solvents	DMSO, water (pH ≥ 5)
Melting Point	>300 °C
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
Smiles	OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
InChi Key	YILMHDCPZJTMGI-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H315, H319, H335
Precautionary statements	P261, P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) Bioconjugate Chem. 6, 447 (1995)
InChi	InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27)
Quantity	50 mg, 200 mg, 1 g, Bulk