Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Yellow solid
Properties
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
- Melting Point
- >300 °C
- Solvents
- DMSO, water (pH ≥ 5)
Available from stock
6-FAM
- SKU
- C0016
Category: Fluorescent Detection
- Synonyms
- 6-Carboxyfluorescein
- 3301-79-9
- CAS-Number
- C21H12O7
- Molecular Formula
- 376.32
- Molecular Weight
- Long Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P261, P305 + P351 + P338
- Hazard statements
- H315, H319, H335
- Description
- The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
- InChi
- InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27)
- References
- (1) Bioconjugate Chem. 6, 447 (1995)
- InChi Key
- YILMHDCPZJTMGI-UHFFFAOYSA-N
- Smiles
- OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
Additional information
| Synonyms | 6-Carboxyfluorescein |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow solid |
| CAS-Number | 3301-79-9 |
| Molecular Formula | C21H12O7 |
| Molecular Weight | 376.32 |
| Identity | 1H-NMR |
| Solvents | DMSO, water (pH ≥ 5) |
| Melting Point | >300 °C |
| Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 |
| Smiles | OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| InChi Key | YILMHDCPZJTMGI-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) Bioconjugate Chem. 6, 447 (1995) |
| InChi | InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27) |
| Quantity | 50 mg, 200 mg, 1 g, Bulk |
