- Synonyms: 6-Carboxyfluorescein
- Purity: ≥98% (HPLC)
- Appearance: Yellow solid.
- CAS-Number: 3301-79-9
- Molecular Formula: C21H12O7
- Molecular Weight: 376.32
- Identity: 1H-NMR
- Solvents: DMSO, water (pH ≥ 5)
- Melting Point: >300 °C
- Fluorescence: λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
- Smiles: OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
- InChi: InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)
- InChi Key: YILMHDCPZJTMGI-UHFFFAOYSA-N
Available from stock
6-FAM
SKU: C0016
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: +4°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
- Description: The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
- References: (1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997), (3) S. K. Lau, et al.; J. Chromatogr. A. 809, 203 (1998), (4) D.A. Fulcher & S.W. J. Wong, (5) S. Nampalli, et al.; Bioconjugate Chem. 13, 468 (2002), (6) L. Milroy, et al.; JACS 134, 8480 (2012), Immunol. Cell Biol. 77, 559 (1999)
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
Additional information
| Melting Point | >300 °C |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Identity | 1H-NMR |
| Availability | 1 |
| CAS-Number | 3301-79-9 |
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 |
| InChi | InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32) |
| Appearance | Yellow solid. |
| InChi Key | YILMHDCPZJTMGI-UHFFFAOYSA-N |
| Long Term Storage | +4°C |
| Quantity | 1 g, 200 mg, 50 mg, Bulk |
| Pricetable | |
| Molecular Formula | |
| References | (1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997), (3) S. K. Lau, et al.; J. Chromatogr. A. 809, 203 (1998), (4) D.A. Fulcher & S.W. J. Wong, Immunol. Cell Biol. 77, 559 (1999), (5) S. Nampalli, et al.; Bioconjugate Chem. 13, 468 (2002), (6) L. Milroy, et al.; JACS 134, 8480 (2012) |
| Shipping | AMBIENT |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Purity | |
| Short Term Storage | +4°C |
| Molecular Weight | |
| Solvents | DMSO, water (pH ≥ 5) |
| Synonyms | 6-Carboxyfluorescein |
| Smiles | OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
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