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    6-FAM

    Available from stock

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      6-FAM

      SKU
      C0016

      Category: Fluorescent Detection

      Synonyms
      6-Carboxyfluorescein
      3301-79-9
      CAS-Number
      C21H12O7
      Molecular Formula
      376.32
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (HPLC)
      Identity
      1H-NMR
      Appearance
      Yellow solid

      Properties

      Fluorescence
      λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
      Melting Point
      >300 °C
      Solvents
      DMSO, water (pH ≥ 5)

      Downloads

      Safety Data Sheet
      CDX C0016 MSDS.pdf
      Long Term Storage
      +4°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at +4°C.
      Handling Advice
      Protect from light and moisture.
      Short Term Storage
      +4°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Signal word
      Warning
      GHS Symbol
      GHS07
      Precautionary statements
      P261, P305 + P351 + P338
      Hazard statements
      H315, H319, H335
      Description
      The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
      InChi
      InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27)
      References
      (1) Bioconjugate Chem. 6, 447 (1995)
      InChi Key
      YILMHDCPZJTMGI-UHFFFAOYSA-N
      Smiles
      OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12

      The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.

      SKU: C0016 Category: Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      6-Carboxyfluorescein

      Purity

      ≥98% (HPLC)

      Appearance

      Yellow solid

      CAS-Number

      3301-79-9

      Molecular Formula

      C21H12O7

      Molecular Weight

      376.32

      Identity

      1H-NMR

      Solvents

      DMSO, water (pH ≥ 5)

      Melting Point

      >300 °C

      Fluorescence

      λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0

      Smiles

      OC(=O)C1=CC=C(C(O)=O)C(=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12

      InChi Key

      YILMHDCPZJTMGI-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      +4°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at +4°C.

      Hazard statements

      H315, H319, H335

      Precautionary statements

      P261, P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) Bioconjugate Chem. 6, 447 (1995)

      InChi

      InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)16-7-10(20(24)25)1-4-13(16)21(26)27/h1-9,22H,(H,24,25)(H,26,27)

      Quantity

      50 mg, 200 mg, 1 g, Bulk

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