- ≥98% (TLC)
- Light red powder
- λex 408 nm, λem 450 nm in methanol
- Melting Point
- ≥250°C (lit.)
- alcohols, DMF
Category: Fluorescent Detection
Phenolic dyes with high pKas – such as 7-hydroxycoumarin (often called umbelliferone) and the more common 7-hydroxy-4-methylcoumarin – are not fully deprotonated and therefore not fully fluorescent unless the pH of the reaction mixture is raised to above pH ~10. Thus, substrates derived from these fluorophores are seldom used for continuous measurement of enzymatic activity in solution or live cells. The similar 3-cyano-7-hydroxycoumarin and 6,8-difluoro-7-hydroxy-4-methylcoumarin have lower pKas, making them suitable for a broader range of applications. Moreover, the -trifluoromethylumbelliferone product has longer-wavelength spectra and stronger absorbance than -methylumbelliferone. Ether, ester and phosphate substrates derived from these phenolic dyes may be fluorescent but invariably exhibit shorter-wavelength absorption and emission spectra.
Light red powder
λex 408 nm, λem 450 nm in methanol
|Short Term Storage|
|Long Term Storage|
Protect from light and moisture.
|Use / Stability|
Stable for at least 2 years after receipt when stored at +4°C.
H302, H312, H315, H319, H332, H335
P261, P280, P305 + P351 + P338
Not dangerous goods
(1) I.N. White et al.: Anal. Biochem. 172(2), 304 (1988)
100 mg, 1 g, 5 g, Bulk