- Synonyms: Demecolcine, N-Deacetyl-N-methylcolchicine
- Purity: ≥98% (HPLC)
- Appearance: Light yellow powder.
- CAS-Number: 477-30-5
- Molecular Formula: C21H25NO5
- Molecular Weight: 371.43
- Identity: 1H-NMR
- Solvents: chloroform (10 mg/ml), ethanol
- Melting Point: 73-75°C
- Smiles: CNC1CCC2=CC(OC)=C(OC)C(OC)=C2C2=CC=C(OC)C(=O)C=C12
- InChi: InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
- InChi Key: NNJPGOLRFBJNIW-UHFFFAOYSA-N
Available from stock
Colcemid
SKU: C0022
Category: Other Pollutants
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: Depolymerizes microtubules and blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
- References: (1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997), (3) T.S. Seo, et al.; J. Org. Chem. 68, 609 (2003), (4) K. Tanaka, et al.; JACS 129, 5612 (2007), (5) G.V. Los, et al.; ACS Chem. Biol. 3, 373 (2008), (6) S. Mizukami, et al.; JACS 134, 1623 (2012)
Depolymerizes microtubules and blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Additional information
| Quantity | 25 mg, Bulk |
|---|---|
| Identity | 1H-NMR |
| Availability | 1 |
| CAS-Number | 477-30-5 |
| InChi | InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2 |
| Molecular Formula | |
| Appearance | Light yellow powder. |
| InChi Key | NNJPGOLRFBJNIW-UHFFFAOYSA-N |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Molecular Weight | |
| Short Term Storage | +4°C |
| Pricetable | |
| References | (1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997), (3) T.S. Seo, et al.; J. Org. Chem. 68, 609 (2003), (4) K. Tanaka, et al.; JACS 129, 5612 (2007), (5) G.V. Los, et al.; ACS Chem. Biol. 3, 373 (2008), (6) S. Mizukami, et al.; JACS 134, 1623 (2012) |
| Shipping | AMBIENT |
| Smiles | CNC1CCC2=CC(OC)=C(OC)C(OC)=C2C2=CC=C(OC)C(=O)C=C12 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Purity | |
| Solvents | chloroform (10 mg/ml), ethanol |
| Synonyms | Demecolcine, N-Deacetyl-N-methylcolchicine |
| Melting Point | 73-75°C |
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