Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Light yellow powder
Properties
- Melting Point
- 73-75°C
- Solvents
- chloroform (10 mg/ml), ethanol
Available from stock
Colcemid
- SKU
- C0022
Category: Other Pollutants
- Synonyms
- Demecolcine , N-Deacetyl-N-methylcolchicine
- 477-30-5
- CAS-Number
- C21H25NO5
- Molecular Formula
- 371.43
- Molecular Weight
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Long Term Storage
- -20°C
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Excepted Quantities
- RIDADR
- UN2811 6.1
- Signal word
- Danger
- GHS Symbol
- GHS06
- Precautionary statements
- P264, P301 + P310
- Hazard statements
- H300
- Description
- Depolymerizes microtubules and blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
- InChi
- InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
- References
- (1) K. Ray et al.: Eur. J. Biochem. 142, 577 (1984)
- InChi Key
- NNJPGOLRFBJNIW-UHFFFAOYSA-N
- Smiles
- CNC1CCC2=CC(OC)=C(OC)C(OC)=C2C2=CC=C(OC)C(=O)C=C12
Depolymerizes microtubules and blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Additional information
| Synonyms | Demecolcine, N-Deacetyl-N-methylcolchicine |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Light yellow powder |
| CAS-Number | 477-30-5 |
| Molecular Formula | C21H25NO5 |
| Molecular Weight | 371.43 |
| Identity | 1H-NMR |
| Solvents | chloroform (10 mg/ml), ethanol |
| Melting Point | 73-75°C |
| Smiles | CNC1CCC2=CC(OC)=C(OC)C(OC)=C2C2=CC=C(OC)C(=O)C=C12 |
| InChi Key | NNJPGOLRFBJNIW-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H300 |
| Precautionary statements | P264, P301 + P310 |
| GHS Symbol | GHS06 |
| Signal word | Danger |
| RIDADR | UN2811 6.1 |
| Transportation | Excepted Quantities |
| References | (1) K. Ray et al.: Eur. J. Biochem. 142, 577 (1984) |
| InChi | InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3 |
| Quantity | 25 mg, Bulk |
