Available from stock

7-Amino-4-trifluoromethylcoumarin

SKU: A0029

Category: Fluorescent Detection

Synonyms: Coumarin 151, AFC; 53518-15-3
CAS-Number: C₁₀H₆F₃NO₂
Molecular Formula: 229.16
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥99% (GC)
Appearance: Yellow solid
Identity: 1H-NMR

Properties

Solvents: Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water.
Melting Point: 221-222 °C (lit.)

Documentation

Safety Data Sheet (SDS): CDX A0029 SDS_Vs1.1.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Hazard statements: H315 - H319 - H335
Precautionary statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: 7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: ?ex 376nm, ?em 482nm, Quantum yield 0.531.
Smiles: NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1
InChi Key: JBNOVHJXQSHGRL-UHFFFAOYSA-N
References: (1) K.H. Drexhage, et al., Opt. Commun. 15, 399 (1975) , (2) R.E. Smith, et al., Thromb. Res. 17, 393 (1980) , (3) E.R. Bissell, et al., J. Org. Chem. 45, 2283 (1980) , (4) R.E. Smith, et al., Histochem. J. 24, 637 (1992) , (5) G. Nowak, J. Biol. Chem. 277, 43377 (2002) , (6) C.-S. Chu & Y.-L. Lo, Sensors and Actuators B: Chemical. 134, 711 (2008) , (67) V. Stanisic, et al., J. Bio. Chem. 284, 16135 (2009) , (8) J.-G. Lee, et al., J. Bio.. Chem. 289, 3510 (2014)

7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: ?ex 376nm, ?em 482nm, Quantum yield 0.531.

SKU: A0029 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Coumarin 151, AFC
Purity	≥99% (GC)
Appearance	Yellow solid
CAS-Number	53518-15-3
Molecular Formula	C10H6F3NO2
Molecular Weight	229.16
Identity	1H-NMR
Solvents	Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water.
Melting Point	221-222 °C (lit.)
Smiles	NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1
InChi Key	JBNOVHJXQSHGRL-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H315 – H319 – H335
Precautionary statements	P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) K.H. Drexhage, et al., Opt. Commun. 15, 399 (1975), (2) R.E. Smith, et al., Thromb. Res. 17, 393 (1980), (3) E.R. Bissell, et al., J. Org. Chem. 45, 2283 (1980), (4) R.E. Smith, et al., Histochem. J. 24, 637 (1992), (5) G. Nowak, J. Biol. Chem. 277, 43377 (2002), (6) C.-S. Chu & Y.-L. Lo, Sensors and Actuators B: Chemical. 134, 711 (2008), (67) V. Stanisic, et al., J. Bio. Chem. 284, 16135 (2009), (8) J.-G. Lee, et al., J. Bio.. Chem. 289, 3510 (2014)
Quantity	1 g, Bulk