Specifications
- Purity
- ≥99% (GC)
- Appearance
- Yellow solid
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water.
- Melting Point
- 221-222 °C (lit.)
Category: Fluorescent Detection, Fluorescent Detection
7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: λex 376nm, λem 482nm, Quantum yield 0.531.
Synonyms | Coumarin 151, AFC |
---|---|
Purity | ≥99% (GC) |
Appearance | Yellow solid |
CAS-Number | 53518-15-3 |
Molecular Formula | C10H6F3NO2 |
Molecular Weight | 229.16 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water. |
Melting Point | 221-222 °C (lit.) |
Smiles | NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1 |
InChi Key | JBNOVHJXQSHGRL-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
Hazard statements | H315 – H319 – H335 |
Precautionary statements | P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) K.H. Drexhage, et al., Opt. Commun. 15, 399 (1975), (2) R.E. Smith, et al., Thromb. Res. 17, 393 (1980), (3) E.R. Bissell, et al., J. Org. Chem. 45, 2283 (1980), (4) R.E. Smith, et al., Histochem. J. 24, 637 (1992), (5) G. Nowak, J. Biol. Chem. 277, 43377 (2002), (6) C.-S. Chu & Y.-L. Lo, Sensors and Actuators B: Chemical. 134, 711 (2008), (67) V. Stanisic, et al., J. Bio. Chem. 284, 16135 (2009), (8) J.-G. Lee, et al., J. Bio.. Chem. 289, 3510 (2014) |
Quantity | 1 g, Bulk |