Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Melting Point
- 110 °C
Documentation
- Safety Data Sheet (SDS)
- CDX A0069 MSDS.pdf
Available from stock
7-Azido-4-methylcoumarin
- SKU
- A0069
Category: Fluorescent Detection
- Synonyms
- 7-Azido-4-methyl-1-benzopyran-2-one , AzMC
- 95633-27-5
- CAS-Number
- C10H7N3O2
- Molecular Formula
- 201.18
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- 7-Azido-4-methylcoumarin is a highly sensitive and selective fluorogenic H2S probe. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with ?ex = 365 nm and ?em = 450 nm. It is a photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1). 7-Azido-4-methylcoumarin is a tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine ?-synthase (CBS), cystathionine ?-lyase (CGL) and tryptophan synthase (TS)) and to identify novel cystathionine ?-synthase (CBS) inhibitors and activators.
- Smiles
- O=C1C=C(C)C2=CC=C(N=[N+]=[N-])C=C2O1
- InChi Key
- HEKDKVLIMUWRRZ-UHFFFAOYSA-N
- References
- (1) G. Chen, et al.: Protein Sci. 8, 2151 (1999) , (2) M.K. Thorson, et al.: Angew. Chem. Int. Ed. Engl. 52, 4641 (2013) , (3) B. Chen, et al.: Analyst. 138, 946 (2013)
- InChi
- InChI=1S/C10H7N3O2/c1-6-4-10(14)15-9-5-7(12-13-11)2-3-8(6)9/h2-5H,1H3
7-Azido-4-methylcoumarin is a highly sensitive and selective fluorogenic H2S probe. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with ?ex = 365 nm and ?em = 450 nm. It is a photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1). 7-Azido-4-methylcoumarin is a tool for monitoring the activity of pyridoxal-5′-phosphate (PLP)-dependent enzymes (e.g. cystathionine ?-synthase (CBS), cystathionine ?-lyase (CGL) and tryptophan synthase (TS)) and to identify novel cystathionine ?-synthase (CBS) inhibitors and activators.
Additional information
| Synonyms | 7-Azido-4-methyl-1-benzopyran-2-one, AzMC |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 95633-27-5 |
| Molecular Formula | C10H7N3O2 |
| Molecular Weight | 201.18 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Melting Point | 110 °C |
| Smiles | O=C1C=C(C)C2=CC=C(N=[N+]=[N-])C=C2O1 |
| InChi Key | HEKDKVLIMUWRRZ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G. Chen, et al.: Protein Sci. 8, 2151 (1999), (2) M.K. Thorson, et al.: Angew. Chem. Int. Ed. Engl. 52, 4641 (2013), (3) B. Chen, et al.: Analyst. 138, 946 (2013) |
| InChi | InChI=1S/C10H7N3O2/c1-6-4-10(14)15-9-5-7(12-13-11)2-3-8(6)9/h2-5H,1H3 |
| Quantity | 1 mg, 10 mg, 50 mg, Bulk |
