- Purity: ≥96% (HPLC)
- Appearance: Yellow powder
- CAS-Number: 139346-57-9
- Molecular Formula: C18H18N2O6
- Molecular Weight: 358.35
- Identity: 1H-NMR
- Solvents: DMSO, DMF or acetonitrile
- Fluorescence: λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0
- Smiles: CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
- InChi: InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3
- InChi Key: NUNPVRICKDZFLK-UHFFFAOYSA-N
Available from stock
7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester
SKU: D0037
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.
- References: (1) B. Steinberg et al.; 104(22), 9523 (2007), (2) A.K. Bhunia et al.; Chem. Biol. Chem. 8(14), 1642 (2007), (3) T. Higashijima et al.; Anal. Chem. 64, 711 (1992)
Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.
Additional information
| Quantity | 1 g, 100 mg, Bulk |
|---|---|
| Fluorescence | λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0 |
| Availability | 1 |
| CAS-Number | 139346-57-9 |
| Identity | 1H-NMR |
| Long Term Storage | -20°C |
| Appearance | Yellow powder |
| InChi | InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3 |
| InChi Key | NUNPVRICKDZFLK-UHFFFAOYSA-N |
| Handling Advice | Protect from light and moisture. |
| Shipping | AMBIENT |
| Molecular Formula | |
| Purity | |
| Pricetable | |
| References | (1) B. Steinberg et al.; 104(22), 9523 (2007), (2) A.K. Bhunia et al.; Chem. Biol. Chem. 8(14), 1642 (2007), (3) T. Higashijima et al.; Anal. Chem. 64, 711 (1992) |
| Short Term Storage | +4°C |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Smiles | CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2 |
| Solvents | DMSO, DMF or acetonitrile |
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