Specifications
- Purity
- ≥96% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Fluorescence
- λex 445 nm, λem 482 nm in 0.1 M phosphate pH 7.0
- Solvents
- DMSO, DMF, acetonitrile
Available from stock
7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester
- SKU
- D0037
Categories: Fluorescent Detection, Fluorescent Detection
- 139346-57-9
- CAS-Number
- C18H18N2O6
- Molecular Formula
- 358.35
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.
- InChi
- InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3
- References
- (1) B. Steinberg et al.: 104(22), 9523 (2007) , (2) A.K. Bhunia et al.: Chem. Biol. Chem. 8(14), 1642 (2007) , (3) T. Higashijima et al.: Anal. Chem. 64, 711 (1992)
- InChi Key
- NUNPVRICKDZFLK-UHFFFAOYSA-N
- Smiles
- CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.
Additional information
| Purity | ≥96% (HPLC) |
|---|---|
| Appearance | Yellow powder |
| CAS-Number | 139346-57-9 |
| Molecular Formula | C18H18N2O6 |
| Molecular Weight | 358.35 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, acetonitrile |
| Fluorescence | λex 445 nm, λem 482 nm in 0.1 M phosphate pH 7.0 |
| Smiles | CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2 |
| InChi Key | NUNPVRICKDZFLK-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) B. Steinberg et al.: 104(22), 9523 (2007), (2) A.K. Bhunia et al.: Chem. Biol. Chem. 8(14), 1642 (2007), (3) T. Higashijima et al.: Anal. Chem. 64, 711 (1992) |
| InChi | InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3 |
| Quantity | 100 mg, 1 g, Bulk |
