7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester

Specifications

Purity

≥96% (HPLC)

Appearance

Yellow powder

Identity

1H-NMR

Properties

Fluorescence

λex 445 nm, λem 482 nm in 0.1 M phosphate pH 7.0

Solvents

DMSO, DMF, acetonitrile

Short Term Storage

+4°C

Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.

Handling Advice

Protect from light and moisture.

Long Term Storage

-20°C

Shipping

AMBIENT

Transportation

Not dangerous goods

Description

Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.

InChi

InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3

References

(1) B. Steinberg et al.: 104(22), 9523 (2007) , (2) A.K. Bhunia et al.: Chem. Biol. Chem. 8(14), 1642 (2007) , (3) T. Higashijima et al.: Anal. Chem. 64, 711 (1992)

InChi Key

NUNPVRICKDZFLK-UHFFFAOYSA-N

Smiles

CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2