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    7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester

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      7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester

      SKU
      D0037

      Categories: Fluorescent Detection, Fluorescent Detection

      139346-57-9
      CAS-Number
      C18H18N2O6
      Molecular Formula
      358.35
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥96% (HPLC)
      Appearance
      Yellow powder
      Identity
      1H-NMR

      Properties

      Fluorescence
      λex 445 nm, λem 482 nm in 0.1 M phosphate pH 7.0
      Solvents
      DMSO, DMF, acetonitrile
      Short Term Storage
      +4°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Long Term Storage
      -20°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.
      InChi
      InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3
      References
      (1) B. Steinberg et al.: 104(22), 9523 (2007) , (2) A.K. Bhunia et al.: Chem. Biol. Chem. 8(14), 1642 (2007) , (3) T. Higashijima et al.: Anal. Chem. 64, 711 (1992)
      InChi Key
      NUNPVRICKDZFLK-UHFFFAOYSA-N
      Smiles
      CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2

      Derivatives of 7-aminocoumarins are the most extensively utilized labeling reagents for preparing brightly blue fluorescent conjugates of proteins and nucleic acid. Labeled amino acids are detected with visible semiconductor laser fluorometry using second harmonic emission (415 nm) of the near-infrared semiconductor laser. Arginine, proline, serine and glycine have been detected with detection limits around 100 amol levels. The fluorescent-labelling better matches the excitation-wavelength of standard blue lasers than conventional fluorescein-isothiocyyanate.

      SKU: D0037 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Purity

      ≥96% (HPLC)

      Appearance

      Yellow powder

      CAS-Number

      139346-57-9

      Molecular Formula

      C18H18N2O6

      Molecular Weight

      358.35

      Identity

      1H-NMR

      Solvents

      DMSO, DMF, acetonitrile

      Fluorescence

      λex 445 nm, λem 482 nm in 0.1 M phosphate pH 7.0

      Smiles

      CCN(CC)C1=CC2=C(C=C1)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2

      InChi Key

      NUNPVRICKDZFLK-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) B. Steinberg et al.: 104(22), 9523 (2007), (2) A.K. Bhunia et al.: Chem. Biol. Chem. 8(14), 1642 (2007), (3) T. Higashijima et al.: Anal. Chem. 64, 711 (1992)

      InChi

      InChI=1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3

      Quantity

      100 mg, 1 g, Bulk

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