Available from stock

Alcian Blue 8GX solution

SKU: A0201

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: Ingrain Blue 1 , Alcian Blue , 75881-23-1 , Alcian Blue 8GS; 33864-99-2
CAS-Number: C₅₆H₆₈Cl₄CuN₁₆S₄
Molecular Formula: 1298.87
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥50% (Dye content) (UV/Vis)
Appearance: Blue liquid
Identity: UV/Vis
Concentration: 1% in solution in 3% acetic acid

Properties

Solvents: water, acetic acid

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: Alcian blue 8GX is primarily used for demonstrating acid mucopolysaccharides, which it does quite selectively. In recent years there has been some difficulty obtaining the dye. Alcian Blue 8GX is also suitable for detection of glycoproteins on nitrocellulose and in PAGE gels. There are other dyes similar to alcian blue, although not necessarily belonging to the same class of dyes. Alcian green has gained some popularity, and alcian yellow has been suggested, especially in a method for demonstrating Helicobacter. They are used for acid mucopolysaccharides in the same way as alcian blue. They have no particular advantage for demonstrating mucin. According to the Merck Index the groups marked X split off during staining.
Smiles: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(SCC1=CC2=C(C=C1)C1=NC3=C4C=C(CSC(N(C)C)=[N+](C)C)C=CC4=C4N=C5/N=C(/N=C6N([Cu]N34)C(=N/C2=N1)C1=C6C=C(CSC(N(C)C)=[N+](C)C)C=C1)C1=C5C=C(CSC(N(C)C)=[N+](C)C)C=C1)=[N+](C)C
InChi Key: XRWQTDIELZDCPS-UHFFFAOYSA-J
References: (1) S. Mozersky et al.: J. Am. Leather Chem. Assoc. 98(9), 337 (2003) , (2) M. Hattori et al.: Bunseki Kagaku 52(4), 259 (2003) , (3) P. Whiteman et al.: Biochem. J. 131(2), 343 (1973)
InChi: InChI=1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-59-45(37)57-46-39-23-19-35(31-75-55(69(9)10)70(11)12)27-43(39)51(61-46)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(62-52)58-47-38-22-18-34(26-42(38)50(60-47)63-49)30-74-54(67(5)6)68(7)8

Alcian blue 8GX is primarily used for demonstrating acid mucopolysaccharides, which it does quite selectively. In recent years there has been some difficulty obtaining the dye. Alcian Blue 8GX is also suitable for detection of glycoproteins on nitrocellulose and in PAGE gels. There are other dyes similar to alcian blue, although not necessarily belonging to the same class of dyes. Alcian green has gained some popularity, and alcian yellow has been suggested, especially in a method for demonstrating Helicobacter. They are used for acid mucopolysaccharides in the same way as alcian blue. They have no particular advantage for demonstrating mucin. According to the Merck Index the groups marked X split off during staining.

SKU: A0201 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	Ingrain Blue 1, Alcian Blue, 75881-23-1, Alcian Blue 8GS
Purity	≥50% (Dye content) (UV/Vis)
Appearance	Blue liquid
CAS-Number	33864-99-2
Molecular Formula	C56H68Cl4CuN16S4
Molecular Weight	1298.87
Identity	UV/Vis
Solvents	water, acetic acid
Smiles	[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(SCC1=CC2=C(C=C1)C1=NC3=C4C=C(CSC(N(C)C)=[N+](C)C)C=CC4=C4N=C5/N=C(/N=C6N([Cu]N34)C(=N/C2=N1)C1=C6C=C(CSC(N(C)C)=[N+](C)C)C=C1)C1=C5C=C(CSC(N(C)C)=[N+](C)C)C=C1)=[N+](C)C
InChi Key	XRWQTDIELZDCPS-UHFFFAOYSA-J
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) S. Mozersky et al.: J. Am. Leather Chem. Assoc. 98(9), 337 (2003), (2) M. Hattori et al.: Bunseki Kagaku 52(4), 259 (2003), (3) P. Whiteman et al.: Biochem. J. 131(2), 343 (1973)
InChi	InChI=1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-59-45(37)57-46-39-23-19-35(31-75-55(69(9)10)70(11)12)27-43(39)51(61-46)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(62-52)58-47-38-22-18-34(26-42(38)50(60-47)63-49)30-74-54(67(5)6)68(7)8
Quantity	500 ml, 2.5 l, Bulk
cdx-Concentration	1% in solution in 3% acetic acid