- Synonyms: BCECF-AM
- Purity: ≥95% (HPCE)
- Appearance: Red oil
- CAS-Number: 117464-70-7
- Molecular Formula: C42H40O21
- Molecular Weight: 808.69
- Identity: 1H-NMR
- Solvents: DMSO, DMF or acetonitrile
- Fluorescence: λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0
- Smiles: CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O
- InChi: InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21
- InChi Key: NTECHUXHORNEGZ-UHFFFAOYSA-N
Available from stock
BCECF-AM
SKU: C0003
Category: Fluorescent Detection
- Shipping: BLUE ICE
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
- References: (1) L. Homolya, et al.; J. Biol. Chem. 268, 21493 (1993), (2) H. Kao, et al.; J. Cell Biol. 120, 175 (1993), (3) K. Fushimi, et al.; J. Cell Biol. 112, 719 (1991), (4) H. Szmacinski, et al.; Analyt. Chem. 65, 1668 (1993)
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
Additional information
| InChi Key | NTECHUXHORNEGZ-UHFFFAOYSA-N |
|---|---|
| Short Term Storage | +4°C |
| Shipping | BLUE ICE |
| Synonyms | BCECF-AM |
| Solvents | DMSO, DMF or acetonitrile |
| Purity | |
| Pricetable | |
| Molecular Weight | |
| Top Sellers | Fluorescent Detection |
| Smiles | CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O |
| References | (1) L. Homolya, et al.; J. Biol. Chem. 268, 21493 (1993), (2) H. Kao, et al.; J. Cell Biol. 120, 175 (1993), (3) K. Fushimi, et al.; J. Cell Biol. 112, 719 (1991), (4) H. Szmacinski, et al.; Analyt. Chem. 65, 1668 (1993) |
| Molecular Formula | |
| Quantity | 1 mg, 5 mg, Bulk |
| Fluorescence | λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 |
| InChi | InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21 |
| Appearance | Red oil |
| Long Term Storage | -20°C |
| CAS-Number | 117464-70-7 |
| Availability | 1 |
| Handling Advice | Protect from light and moisture. |
| Identity | 1H-NMR |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
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