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    BCECF-AM

    Available from stock

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      BCECF-AM

      SKU
      C0003

      Category: Fluorescent Detection

      117464-70-7
      CAS-Number
      C42H40O21
      Molecular Formula
      808.69
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥95% (HPCE)
      Appearance
      Red oil
      Identity
      1H-NMR

      Properties

      Fluorescence
      λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0
      Solvents
      DMSO, DMF, acetonitrile

      Downloads

      Safety Data Sheet
      CDX C0003 MSDS.pdf
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Long Term Storage
      -20°C
      Short Term Storage
      +4°C
      Shipping
      BLUE ICE
      Transportation
      Not dangerous goods
      Signal word
      Warning
      GHS Symbol
      GHS07
      Precautionary statements
      P261, P305 + P351 + P338
      Hazard statements
      H315, H319, H335
      Description
      Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
      InChi
      InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
      References
      (1) Urano et al.: Chem. Lett. 12, 1217 (1997) , (2) W. Obexer et al: Tropical Med. Parasit. 46(1), 45 (1995) , (3) A.K. Ho et al.: Am. J. Physiol. 261, C642 (1991)
      InChi Key
      NTECHUXHORNEGZ-UHFFFAOYSA-N
      Smiles
      CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O

      Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

      SKU: C0003 Category: Fluorescent Detection
      • Additional information

      Additional information

      Purity

      ≥95% (HPCE)

      Appearance

      Red oil

      CAS-Number

      117464-70-7

      Molecular Formula

      C42H40O21

      Molecular Weight

      808.69

      Identity

      1H-NMR

      Solvents

      DMSO, DMF, acetonitrile

      Fluorescence

      λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0

      Smiles

      CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O

      InChi Key

      NTECHUXHORNEGZ-UHFFFAOYSA-N

      Shipping

      BLUE ICE

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Hazard statements

      H315, H319, H335

      Precautionary statements

      P261, P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) Urano et al.: Chem. Lett. 12, 1217 (1997), (2) W. Obexer et al: Tropical Med. Parasit. 46(1), 45 (1995), (3) A.K. Ho et al.: Am. J. Physiol. 261, C642 (1991)

      InChi

      InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3

      Quantity

      1 mg, 5 mg, Bulk

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