Specifications
- Purity
- ≥95% (HPCE)
- Appearance
- Red oil
- Identity
- 1H-NMR
Properties
- Fluorescence
- λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0
- Solvents
- DMSO, DMF, acetonitrile
Available from stock
BCECF-AM
- SKU
- C0003
Category: Fluorescent Detection
- 117464-70-7
- CAS-Number
- C42H40O21
- Molecular Formula
- 808.69
- Molecular Weight
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Long Term Storage
- -20°C
- Short Term Storage
- +4°C
- Shipping
- BLUE ICE
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P261, P305 + P351 + P338
- Hazard statements
- H315, H319, H335
- Description
- Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
- InChi
- InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
- References
- (1) Urano et al.: Chem. Lett. 12, 1217 (1997) , (2) W. Obexer et al: Tropical Med. Parasit. 46(1), 45 (1995) , (3) A.K. Ho et al.: Am. J. Physiol. 261, C642 (1991)
- InChi Key
- NTECHUXHORNEGZ-UHFFFAOYSA-N
- Smiles
- CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
Additional information
| Purity | ≥95% (HPCE) |
|---|---|
| Appearance | Red oil |
| CAS-Number | 117464-70-7 |
| Molecular Formula | C42H40O21 |
| Molecular Weight | 808.69 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, acetonitrile |
| Fluorescence | λex 482 nm, λem 528 nm in 0.1 M Tris pH 8.0 |
| Smiles | CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O |
| InChi Key | NTECHUXHORNEGZ-UHFFFAOYSA-N |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) Urano et al.: Chem. Lett. 12, 1217 (1997), (2) W. Obexer et al: Tropical Med. Parasit. 46(1), 45 (1995), (3) A.K. Ho et al.: Am. J. Physiol. 261, C642 (1991) |
| InChi | InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3 |
| Quantity | 1 mg, 5 mg, Bulk |
