Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- White to off-white solid
Properties
- Solvents
- DMSO
Available from stock
Calcein-AM
- SKU
- C0009
Category: Fluorescent Detection
- Synonyms
- Calcein O,O'-diacetate tetrakis(acetoxymethyl) ester , Calcein acetoxymethyl ester , Diacetate , CAL-AM , NSC 689290
- 148504-34-1
- CAS-Number
- C46H46N2O23
- Molecular Formula
- 994.86
- Molecular Weight
- Long Term Storage
- -20°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- -20°C
- Shipping
- BLUE ICE
- Transportation
- Not dangerous goods
- Description
- Calcein AM is a non-fluorescent cell permeable and hydrophobic probe that, upon entering live cells, is cleaved by intracellular esterases, releasing the membrane-impermeable, hydrophilic, and intensely bright green fluorescent calcein. Better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, due to its low cytotoxicity. It can be used as an indicator of cell viability (dead cells lack cytoplasmic esterases), cell-cell communication, cytotoxicity or changes in intracellular calcium, fluoride, iron or mercury. Calcein-AM is a neutral substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. Viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay. Spectral Data: λex 496nm, λem 516nm in 0.1M Tris pH 8.0.
- InChi
- InChI=1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
- References
- (1) N. Feller, et al., Br. J. Cancer. 72, 543 (1995) , (2) N. Feller, et al., FEBS Lett. 368 385 (1995) , (3) F. Tiberghien & F. Loor, Anti-Cancer Drugs 7, 568 (1996) , (4) P. Decherchi, et al., J. Neurosci. Methods 71, 205 (1997) , (5) M. Essodaigui, et al., Biochemistry 37, 2243 (1998) , (6) J.J. Lemasters, et al., Biochim. Biophys. Acta. 1366, 177 (1998) , (7) J. Hynes, et al., J. Biomol. Screen. 8, 264 (2003) , (8) D. Bratosin, et al., Cytometry A 66, 78 (2005) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010) , (10) G. Szakacs, et al., Pathol. Oncol. Res. 4, 251 (2016)
- InChi Key
- XKFSBWQWNMZWFA-UHFFFAOYSA-N
- Smiles
- O=C(C1=C2C=CC=C1)OC32C4=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OC(C)=O)C=C4)OC5=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=CC=C53
Calcein AM is a non-fluorescent cell permeable and hydrophobic probe that, upon entering live cells, is cleaved by intracellular esterases, releasing the membrane-impermeable, hydrophilic, and intensely bright green fluorescent calcein. Better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, due to its low cytotoxicity. It can be used as an indicator of cell viability (dead cells lack cytoplasmic esterases), cell-cell communication, cytotoxicity or changes in intracellular calcium, fluoride, iron or mercury. Calcein-AM is a neutral substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. Viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay. Spectral Data: λex 496nm, λem 516nm in 0.1M Tris pH 8.0.
Additional information
| Synonyms | Calcein O,O'-diacetate tetrakis(acetoxymethyl) ester, Calcein acetoxymethyl ester, Diacetate, CAL-AM, NSC 689290 |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | White to off-white solid |
| CAS-Number | 148504-34-1 |
| Molecular Formula | C46H46N2O23 |
| Molecular Weight | 994.86 |
| Solvents | DMSO |
| Smiles | O=C(C1=C2C=CC=C1)OC32C4=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OC(C)=O)C=C4)OC5=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=CC=C53 |
| InChi Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Shipping | BLUE ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) N. Feller, et al., Br. J. Cancer. 72, 543 (1995), (2) N. Feller, et al., FEBS Lett. 368 385 (1995), (3) F. Tiberghien & F. Loor, Anti-Cancer Drugs 7, 568 (1996), (4) P. Decherchi, et al., J. Neurosci. Methods 71, 205 (1997), (5) M. Essodaigui, et al., Biochemistry 37, 2243 (1998), (6) J.J. Lemasters, et al., Biochim. Biophys. Acta. 1366, 177 (1998), (7) J. Hynes, et al., J. Biomol. Screen. 8, 264 (2003), (8) D. Bratosin, et al., Cytometry A 66, 78 (2005), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010), (10) G. Szakacs, et al., Pathol. Oncol. Res. 4, 251 (2016) |
| InChi | InChI=1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3 |
| Quantity | 1 mg, Bulk |
