Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Light yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0
Documentation
- Safety Data Sheet (SDS)
- CDX D0084 MSDS.pdf
Available from stock
DAF-2
- SKU
- D0084
Category: Fluorescent Detection
- Synonyms
- 4,5-Diaminofluorescein , 5,6-Diaminofluorescein
- 205391-01-1
- CAS-Number
- C20H14N2O5
- Molecular Formula
- 362.34
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H302
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
- Smiles
- NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
- InChi Key
- LTYUPYUWXRTNFQ-UHFFFAOYSA-N
- References
- (1) M. Kirsch et al.: J. Pineal Res. 40(1), 10 (2006) , (2) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003) , (3) N. Nagata et al.: J. Biochem. 125(4), 658 (1999) , (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998) , (5) H. Kojima et al.: Anal. Chem. 70, 2446 (1998)
- InChi
- InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell’s cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
Additional information
| Synonyms | 4,5-Diaminofluorescein, 5,6-Diaminofluorescein |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Light yellow powder |
| CAS-Number | 205391-01-1 |
| Molecular Formula | C20H14N2O5 |
| Molecular Weight | 362.34 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0 |
| Smiles | NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| InChi Key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) M. Kirsch et al.: J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al.: Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al.: J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al.: Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.: Anal. Chem. 70, 2446 (1998) |
| InChi | InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 |
| Quantity | 1 mg, 5 mg, 10 mg, Bulk |
