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    DAN

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    DAN

    SKU
    D0062

    Category: Fluorescent Detection

    Synonyms
    2,3-Diaminonaphthalene
    771-97-1
    CAS-Number
    C10H10N2
    Molecular Formula
    158.2
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥98% (HPLC)
    Identity
    1H-NMR
    Appearance
    Off-white to light brown solid

    Properties

    Fluorescence
    λex 364 nm, λem 406 nm in 0.5 M Na carbonate pH 10.0 (after derivatization with NaNO2 in 0.1 M HCl)
    Melting Point
    197-203 °C
    Solvents
    DMSO, DMF or pyridine

    Downloads

    Safety Data Sheet
    CDX D0062 MSDS.pdf
    Long Term Storage
    +4°C
    Use / Stability
    Stable for at least 2 years after receipt when stored at +4°C.
    Handling Advice
    Protect from light and moisture.
    Short Term Storage
    +4°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Signal word
    Danger
    GHS Symbol
    GHS07+GHS08
    Precautionary statements
    P201, P261, P305 + P351 + P338, P308 + P313
    Hazard statements
    H302, H315, H319, H335, H350
    Description
    The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.
    InChi
    InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2
    References
    (1) J. Pedro et al.: Anal. Chim. Acta 516(1-2), 229 (2004) , (2) T. Nagano et al.: Biol. Pharm. Bull. 21(12), 1247 (1998) , (3) W.C. Hawkes et al.: Anal. Biochem. 241(2), 206 (1996) , (4) R.F. Bayfield et al.: Anal. Biochem. 144(2), 569 (1995) , (5) P. Damiani et al.: Talanta 33(8), 649 (1986)
    InChi Key
    XTBLDMQMUSHDEN-UHFFFAOYSA-N
    Smiles
    NC1=CC2=CC=CC=C2C=C1N

    The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.

    SKU: D0062 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    2,3-Diaminonaphthalene

    Purity

    ≥98% (HPLC)

    Appearance

    Off-white to light brown solid

    CAS-Number

    771-97-1

    Molecular Formula

    C10H10N2

    Molecular Weight

    158.2

    Identity

    1H-NMR

    Solvents

    DMSO, DMF or pyridine

    Melting Point

    197-203 °C

    Fluorescence

    λex 364 nm, λem 406 nm in 0.5 M Na carbonate pH 10.0 (after derivatization with NaNO2 in 0.1 M HCl)

    Smiles

    NC1=CC2=CC=CC=C2C=C1N

    InChi Key

    XTBLDMQMUSHDEN-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    +4°C

    Long Term Storage

    +4°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at +4°C.

    Hazard statements

    H302, H315, H319, H335, H350

    Precautionary statements

    P201, P261, P305 + P351 + P338, P308 + P313

    GHS Symbol

    GHS07+GHS08

    Signal word

    Danger

    Transportation

    Not dangerous goods

    References

    (1) J. Pedro et al.: Anal. Chim. Acta 516(1-2), 229 (2004), (2) T. Nagano et al.: Biol. Pharm. Bull. 21(12), 1247 (1998), (3) W.C. Hawkes et al.: Anal. Biochem. 241(2), 206 (1996), (4) R.F. Bayfield et al.: Anal. Biochem. 144(2), 569 (1995), (5) P. Damiani et al.: Talanta 33(8), 649 (1986)

    InChi

    InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2

    Quantity

    1 g, 5 g, Bulk

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