DAN

SKU: D0062

Category: Fluorescent Detection

Synonyms: 2,3-Diaminonaphthalene; 771-97-1
CAS-Number: C₁₀H₁₀N₂
Molecular Formula: 158.2
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Off-white to light brown solid

Properties

Fluorescence: λex 364 nm, λem 406 nm in 0.5 M Na carbonate pH 10.0 (after derivatization with NaNO2 in 0.1 M HCl)
Melting Point: 197-203 °C
Solvents: DMSO, DMF or pyridine

Long Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Danger
GHS Symbol: GHS07+GHS08
Precautionary statements: P201, P261, P305 + P351 + P338, P308 + P313
Hazard statements: H302, H315, H319, H335, H350

Description: The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.
InChi: InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2
References: (1) J. Pedro et al.: Anal. Chim. Acta 516(1-2), 229 (2004) , (2) T. Nagano et al.: Biol. Pharm. Bull. 21(12), 1247 (1998) , (3) W.C. Hawkes et al.: Anal. Biochem. 241(2), 206 (1996) , (4) R.F. Bayfield et al.: Anal. Biochem. 144(2), 569 (1995) , (5) P. Damiani et al.: Talanta 33(8), 649 (1986)
InChi Key: XTBLDMQMUSHDEN-UHFFFAOYSA-N
Smiles: NC1=CC2=CC=CC=C2C=C1N

The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.

SKU: D0062 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	2,3-Diaminonaphthalene
Purity	≥98% (HPLC)
Appearance	Off-white to light brown solid
CAS-Number	771-97-1
Molecular Formula	C10H10N2
Molecular Weight	158.2
Identity	1H-NMR
Solvents	DMSO, DMF or pyridine
Melting Point	197-203 °C
Fluorescence	λex 364 nm, λem 406 nm in 0.5 M Na carbonate pH 10.0 (after derivatization with NaNO2 in 0.1 M HCl)
Smiles	NC1=CC2=CC=CC=C2C=C1N
InChi Key	XTBLDMQMUSHDEN-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302, H315, H319, H335, H350
Precautionary statements	P201, P261, P305 + P351 + P338, P308 + P313
GHS Symbol	GHS07+GHS08
Signal word	Danger
Transportation	Not dangerous goods
References	(1) J. Pedro et al.: Anal. Chim. Acta 516(1-2), 229 (2004), (2) T. Nagano et al.: Biol. Pharm. Bull. 21(12), 1247 (1998), (3) W.C. Hawkes et al.: Anal. Biochem. 241(2), 206 (1996), (4) R.F. Bayfield et al.: Anal. Biochem. 144(2), 569 (1995), (5) P. Damiani et al.: Talanta 33(8), 649 (1986)
InChi	InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2
Quantity	1 g, 5 g, Bulk