Specifications
- Purity
- ≥95% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, ethanol, water, chloroform, acetone (25 mg/ml)
- Melting Point
- 200-203°C
Available from stock
Fluorescein diacetate
- SKU
- F0027
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 3,6-Diacetoxyfluoran , Di-O-Acetylfluorescein , Cellstain-FDA , NSC 4726 , NSC 667259
- 596-09-8
- CAS-Number
- C24H16O7
- Molecular Formula
- 416.38
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: ?ex=494nm, ?em=521nm.
- Smiles
- O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC(C)=O)C=C4)OC5=CC(OC(C)=O)=CC=C53
- InChi Key
- CHADEQDQBURGHL-UHFFFAOYSA-N
- References
- (1) B. Rotman, et al. , PNAS 55, 134 (1966) , (2) H.R. Hulett, et al. , Science 166, 747 (1969) , (3) K.H. Jones, et al. , J. Histochem. Cytochem. 33, 77 (1985) , (4) K. McGinnes, et al. , J. Immunol. Methods 86, 7 (1986) , (5) D.D. Ross, et al. , Cancer Res. 49, 3776 (1989) , (6) E. Prosperi , Histochem. J. 22, 227 (1990) , (7) M. Miyamoto, et al. , Cell Transplant 9, 681 (2000) , (8) S. Wanandy, et al. , J. Microbiol. Methods 60, 21 (2005) , (9) J. Wang, et al. , Drug Metab. Dispos. 39, 1329 (2011) , (10) N. Saruyama, et al. , Anal. Biochem. 441, 58 (2013)
FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: ?ex=494nm, ?em=521nm.
Additional information
| Synonyms | 3,6-Diacetoxyfluoran, Di-O-Acetylfluorescein, Cellstain-FDA, NSC 4726, NSC 667259 |
|---|---|
| Purity | ≥95% (NMR) |
| Appearance | White to off-white powder |
| CAS-Number | 596-09-8 |
| Molecular Formula | C24H16O7 |
| Molecular Weight | 416.38 |
| Identity | 1H-NMR |
| Solvents | DMSO, ethanol, water, chloroform, acetone (25 mg/ml) |
| Melting Point | 200-203°C |
| Smiles | O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC(C)=O)C=C4)OC5=CC(OC(C)=O)=CC=C53 |
| InChi Key | CHADEQDQBURGHL-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) B. Rotman, et al., PNAS 55, 134 (1966), (2) H.R. Hulett, et al., Science 166, 747 (1969), (3) K.H. Jones, et al., J. Histochem. Cytochem. 33, 77 (1985), (4) K. McGinnes, et al., J. Immunol. Methods 86, 7 (1986), (5) D.D. Ross, et al., Cancer Res. 49, 3776 (1989), (6) E. Prosperi, Histochem. J. 22, 227 (1990), (7) M. Miyamoto, et al., Cell Transplant 9, 681 (2000), (8) S. Wanandy, et al., J. Microbiol. Methods 60, 21 (2005), (9) J. Wang, et al., Drug Metab. Dispos. 39, 1329 (2011), (10) N. Saruyama, et al., Anal. Biochem. 441, 58 (2013) |
| Quantity | 10 g, 25 g, 100 g, Bulk |
