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    Gram’s Decolorizer Solution

    Available from stock

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      Gram’s Decolorizer Solution

      SKU
      G0062

      Category: Non Fluorescent Detection & Chromogene Detection

      37348-17-7 (comp.)
      CAS-Number
      n/a
      Molecular Formula
      n/a
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      for microscopy
      Appearance
      Colourless liquid
      Identity
      1H-NMR

      Properties

      Density
      0.80 g/ml at 20 °C
      Refractive Index
      n20/D 1.361

      Downloads

      Safety Data Sheet
      CDX G0062 MSDS.pdf
      Use / Stability
      This product should not be used if colour has changed or if there are
      Handling Advice
      Protect from light and moisture.
      Long Term Storage
      RT
      Short Term Storage
      RT
      Shipping
      AMBIENT
      Transportation
      Packing Group II
      RIDADR
      UN 1993BF
      Signal word
      Danger
      GHS Symbol
      GHS02+GHS07
      Precautionary statements
      P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235
      Hazard statements
      H225-H319-H336
      Description
      Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
      References
      (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

      SKU: G0062 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Purity

      for microscopy

      Appearance

      Colourless liquid

      CAS-Number

      37348-17-7 (comp.)

      Molecular Formula

      n/a

      Molecular Weight

      n/a

      Identity

      1H-NMR

      Refractive Index

      n20/D 1.361

      Density

      0.80 g/ml at 20 °C

      Shipping

      AMBIENT

      Short Term Storage

      RT

      Long Term Storage

      RT

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      This product should not be used if colour has changed or if there are

      Hazard statements

      H225-H319-H336

      Precautionary statements

      P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235

      GHS Symbol

      GHS02+GHS07

      Signal word

      Danger

      RIDADR

      UN 1993BF

      Transportation

      Packing Group II

      References

      (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Quantity

      250 ml, 2.5 l, Bulk

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