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    Gram’s Decolorizer Solution

    Available from stock

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      Gram’s Decolorizer Solution

      SKU
      G0062

      Category: Non Fluorescent Detection & Chromogene Detection

      37348-17-7 (comp.)
      CAS-Number
      n/a
      Molecular Formula
      n/a
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      for microscopy
      Appearance
      Colourless liquid
      Identity
      1H-NMR

      Properties

      Refractive Index
      n20/D 1.361
      Density
      0.80 g/ml at 20 °C

      Documentation

      Safety Data Sheet (SDS)
      CDX G0062 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      RT
      Long Term Storage
      RT
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      This product should not be used if colour has changed or if there are
      Hazard statements
      H225-H319-H336
      Precautionary statements
      P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235
      GHS Symbol
      GHS02+GHS07
      Signal word
      Danger
      RIDADR
      UN 1993BF
      Transportation
      Packing Group II
      Description
      Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
      References
      (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

      SKU: G0062 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Purity

      for microscopy

      Appearance

      Colourless liquid

      CAS-Number

      37348-17-7 (comp.)

      Molecular Formula

      n/a

      Molecular Weight

      n/a

      Identity

      1H-NMR

      Refractive Index

      n20/D 1.361

      Density

      0.80 g/ml at 20 °C

      Shipping

      AMBIENT

      Short Term Storage

      RT

      Long Term Storage

      RT

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      This product should not be used if colour has changed or if there are

      Hazard statements

      H225-H319-H336

      Precautionary statements

      P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235

      GHS Symbol

      GHS02+GHS07

      Signal word

      Danger

      RIDADR

      UN 1993BF

      Transportation

      Packing Group II

      References

      (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)

      Quantity

      250 ml, 2.5 l, Bulk

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