Specifications
- Purity
- for microscopy
- Appearance
- Colourless liquid
- Identity
- 1H-NMR
Properties
- Density
- 0.80 g/ml at 20 °C
- Refractive Index
- n20/D 1.361
Available from stock
Gram’s Decolorizer Solution
- SKU
- G0062
Category: Non Fluorescent Detection & Chromogene Detection
- 37348-17-7 (comp.)
- CAS-Number
- n/a
- Molecular Formula
- n/a
- Molecular Weight
- Use / Stability
- This product should not be used if colour has changed or if there are
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- RT
- Short Term Storage
- RT
- Shipping
- AMBIENT
- Transportation
- Packing Group II
- RIDADR
- UN 1993BF
- Signal word
- Danger
- GHS Symbol
- GHS02+GHS07
- Precautionary statements
- P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235
- Hazard statements
- H225-H319-H336
- Description
- Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
- References
- (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)
Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Additional information
| Purity | for microscopy |
|---|---|
| Appearance | Colourless liquid |
| CAS-Number | 37348-17-7 (comp.) |
| Molecular Formula | n/a |
| Molecular Weight | n/a |
| Identity | 1H-NMR |
| Refractive Index | n20/D 1.361 |
| Density | 0.80 g/ml at 20 °C |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | This product should not be used if colour has changed or if there are |
| Hazard statements | H225-H319-H336 |
| Precautionary statements | P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235 |
| GHS Symbol | GHS02+GHS07 |
| Signal word | Danger |
| RIDADR | UN 1993BF |
| Transportation | Packing Group II |
| References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
| Quantity | 250 ml, 2.5 l, Bulk |
