Available from stock

PBFI-AM

SKU: P0068

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: Potassium indicator PBFI-AM , Potassium-binding benzofuran isophthalate-AM , PBFI diacetate; 124549-23-1
CAS-Number: C₅₈H₆₂N₂O₂₄
Molecular Formula: 1171.11
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPCE)
Appearance: Orange powder

Properties

Solvents: DMSO, methanol
Fluorescence: λex 334 nm, λem 525 nm (with Na), λex 370 nm, λem 540 nm in methanol

Shipping: AMBIENT
Short Term Storage: -20°C
Long Term Storage: -20°C
Handling Advice: Very light-sensitive. Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: λex=370nm, λem=540nm in methanol.
Smiles: O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC3=CC(OC)=C(N4CCOCCOCCN(C5=C(OC)C=C6C(OC(C7=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C7)=C6)=C5)CCOCCOCC4)C=C3O2
InChi Key: AUKRBWNSOWVABU-UHFFFAOYSA-N
References: (1) P. Jezek, et al., J. Biol. Chem. 265, 10522 (1990) , (2) S. Lindberg, Planta 195, 525 (1995) , (3) C.D. Bortner, et al., J. Biol. Chem. 272, 32436 (1997) , (4) S.J. Halperin & J.P. Lynch, J. Exp. Bot. 54, 2035 (2003) , (5) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi: InChI=1S/C58H62N2O24/c1-35(61)75-31-79-55(65)39-7-9-43(45(23-39)57(67)81-33-77-37(3)63)51-25-41-27-53(69-5)47(29-49(41)83-51)59-11-15-71-19-21-73-17-13-60(14-18-74-22-20-72-16-12-59)48-30-50-42(28-54(48)70-6)26-52(84-50)44-10-8-40(56(66)80-32-76-36(2)62)24-46(44)58(68)82-34-78-38(4)64/h7-10,23-30H,11-22,31-34H2,1-6H3

PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: λex=370nm, λem=540nm in methanol.

SKU: P0068 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	Potassium indicator PBFI-AM, Potassium-binding benzofuran isophthalate-AM, PBFI diacetate
Purity	≥98% (HPCE)
Appearance	Orange powder
CAS-Number	124549-23-1
Molecular Formula	C58H62N2O24
Molecular Weight	1171.11
Solvents	DMSO, methanol
Fluorescence	λex 334 nm, λem 525 nm (with Na), λex 370 nm, λem 540 nm in methanol
Smiles	O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC3=CC(OC)=C(N4CCOCCOCCN(C5=C(OC)C=C6C(OC(C7=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C7)=C6)=C5)CCOCCOCC4)C=C3O2
InChi Key	AUKRBWNSOWVABU-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Very light-sensitive. Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) P. Jezek, et al., J. Biol. Chem. 265, 10522 (1990), (2) S. Lindberg, Planta 195, 525 (1995), (3) C.D. Bortner, et al., J. Biol. Chem. 272, 32436 (1997), (4) S.J. Halperin & J.P. Lynch, J. Exp. Bot. 54, 2035 (2003), (5) R.W. Sabnis, Handbook of biological dyes and stains (2010)
InChi	InChI=1S/C58H62N2O24/c1-35(61)75-31-79-55(65)39-7-9-43(45(23-39)57(67)81-33-77-37(3)63)51-25-41-27-53(69-5)47(29-49(41)83-51)59-11-15-71-19-21-73-17-13-60(14-18-74-22-20-72-16-12-59)48-30-50-42(28-54(48)70-6)26-52(84-50)44-10-8-40(56(66)80-32-76-36(2)62)24-46(44)58(68)82-34-78-38(4)64/h7-10,23-30H,11-22,31-34H2,1-6H3
Quantity	1 mg, Bulk