Specifications
- Purity
- ≥98% (HPCE)
- Appearance
- Orange powder
Properties
- Solvents
- DMSO, methanol
- Fluorescence
- λex 334 nm, λem 525 nm (with Na), λex 370 nm, λem 540 nm in methanol
Category: Fluorescent Detection, Fluorescent Detection
PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: λex=370nm, λem=540nm in methanol.
Synonyms | Potassium indicator PBFI-AM, Potassium-binding benzofuran isophthalate-AM, PBFI diacetate |
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Purity | ≥98% (HPCE) |
Appearance | Orange powder |
CAS-Number | 124549-23-1 |
Molecular Formula | C58H62N2O24 |
Molecular Weight | 1171.11 |
Solvents | DMSO, methanol |
Fluorescence | λex 334 nm, λem 525 nm (with Na), λex 370 nm, λem 540 nm in methanol |
Smiles | O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC3=CC(OC)=C(N4CCOCCOCCN(C5=C(OC)C=C6C(OC(C7=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C7)=C6)=C5)CCOCCOCC4)C=C3O2 |
InChi Key | AUKRBWNSOWVABU-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice | Very light-sensitive. Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) P. Jezek, et al., J. Biol. Chem. 265, 10522 (1990), (2) S. Lindberg, Planta 195, 525 (1995), (3) C.D. Bortner, et al., J. Biol. Chem. 272, 32436 (1997), (4) S.J. Halperin & J.P. Lynch, J. Exp. Bot. 54, 2035 (2003), (5) R.W. Sabnis, Handbook of biological dyes and stains (2010) |
InChi | InChI=1S/C58H62N2O24/c1-35(61)75-31-79-55(65)39-7-9-43(45(23-39)57(67)81-33-77-37(3)63)51-25-41-27-53(69-5)47(29-49(41)83-51)59-11-15-71-19-21-73-17-13-60(14-18-74-22-20-72-16-12-59)48-30-50-42(28-54(48)70-6)26-52(84-50)44-10-8-40(56(66)80-32-76-36(2)62)24-46(44)58(68)82-34-78-38(4)64/h7-10,23-30H,11-22,31-34H2,1-6H3 |
Quantity | 1 mg, Bulk |