Specifications
- Purity
- ≥98% (TLC)
- Appearance
- Orange to dark orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO (5mg/ml) or DMF (20mg/ml).
- Melting Point
- 223 °C (lit.)
- Fluorescence
- 593.0 ± 5.0 nm
Category: Fluorescent Detection, Fluorescent Detection
Resorufin acetate is fluorogenic substrate for hydrolytic esterases and cellulases used for monitoring cytosolic aldehyde dehydrogenase (ALDH1A1) esterase activity, chymotrypsin activity. Resorufin acetate has been used as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase (GOD), without significant effects of ascorbic acid, uric acid, or bilirubin. It also has been used for the chromogenic and fluorescent detection of hydrazine, acyl protein thioesterase (APT) enzymes and perborate ions, and reveals a selective turn-on type chromogenic and fluorogenic signaling behavior based on the selective and efficient cleavage of acetate group. Upon enzymatic cleavage of the acetate group of resorufin acetate, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=585nm.
Purity | ≥98% (TLC) |
---|---|
Appearance | Orange to dark orange powder |
CAS-Number | 1152-14-3 |
Molecular Formula | C14H9NO4 |
Molecular Weight | 255.23 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO (5mg/ml) or DMF (20mg/ml). |
Melting Point | 223 °C (lit.) |
Fluorescence | 593.0 ± 5.0 nm |
Smiles | O=C1C=C2OC3=CC(OC(C)=O)=CC=C3N=C2C=C1 |
InChi Key | UJWKHDKBOVPINX-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H302 + H332 – H315 – H319 |
Precautionary statements | P261 – P264 – P301 + P312 – P302 + P352 – P304 + P340 + P312 – P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) G.G. Guilbault & A.N.J. Heyn, Anal. Lett. 1, 163 (1967), (2) T.M. Kitson & K.E. Kitson, Biochem J. 322, 701 (1997), (3) T.M. Kitson & K.E. Kitson, Adv. Exp. Med. Biol. 414, 201 (1997), (4) T.M. Kitson, Biochim. Biophys. Acta 1385, 43 (1998), (5) H. Maeda, et al., Chem. Pharm. Bull. 49, 294 (2001), (6) M.G. Choi, et al., Org. Lett. 12, 1468 (2010), (7) M.G. Choi, et al., Org. Biomol. Chem. 11, 2961 (2013) |
Quantity | 5 mg, 25 mg, Bulk |