Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Orange to red powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO.
Category: Fluorescent Detection, Fluorescent Detection
Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 µM to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=585nm.
Synonyms | Benzyloxyresorufin, 7-Benzyloxyresorufin, O7-Benzylresorufin, 7-Benzyloxy-3H-phenoxazin-3-one |
---|---|
Purity | ≥98% (HPLC) |
Appearance | Orange to red powder |
CAS-Number | 87687-02-3 |
Molecular Formula | C19H13NO3 |
Molecular Weight | 303.31 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO. |
Smiles | O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3N=C2C=C1 |
InChi Key | XNZRYTITWLGTJS-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H315 – H319 – H335 |
Precautionary statements | P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) R.T Mayer, at al., Biochem. Pharmacol. 40, 1645 (1990), (2) D.M. Stresser, et al., Drug Metab. Dispos. 28, 1440 (2000), (3) A.B. Renwick, et al., Xenobiotica 31, 861 (2001), (4) D.M. Stresser, et al., Drug Metab. Dispos. 30, 845 (2002), (5) B.M.A. Lussenburg, et at., Anal. Biochem. 341, 148 (2005), (6) J.R. Reed, et al., Biochem. Pharmacol. 95, 126 (2015), (7) J. Reinen, et al., J. Biomol. Screen. 20, 1246 (2015), (8) M.J. Traylor, et al., Insect Biochem. Mol. Biol. 90, 14 (2017), (9) L. Rutz, et al., Ach. Toxicol. 94, 4159 (2020) |
Quantity | 5 mg, 10 mg, Bulk |