Specifications
- Purity
- ≥80% (HPLC)
- Appearance
- Red-purple solid
Properties
- Solvents
- DMSO, DMF, methanol, chloroform
Documentation
- Safety Data Sheet (SDS)
- CDX A0072 MSDS.pdf
Available from stock
Rhod-2 AM
- SKU
- A0072
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 129787-64-0
- 145037-81-6
- CAS-Number
- C52H59BrN4O19
- Molecular Formula
- 1123.96
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- -20°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluoregenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: ?ex 550nm, ?em 578nm in methanol, λex 549nm, λem 578nm in DMSO, λex 550nm, λem 570nm in Chloroform, Methanol (90:10)
- Smiles
- O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=C(C=C(C=C1)C2=C3C=C/C(C=C3OC4=CC(N(C)C)=CC=C24)=[N+](C)/C)OCCOC5=C(C=CC(C)=C5)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O.[Br-]
- InChi Key
- RWXWZOWDWYQKBK-UHFFFAOYSA-M
- References
- (1) A. Minta, et al., J. Biol. Chem. 264, 8171 (1989) , (2) T.M. Quinton, et al., BBRC 184, 893 (1992) , (3) M. Okada, et al., Brain Res. 583, 227 (1992) , (4) Y. Tamura, et al., Brain Res. 592, 317 (1992) , (5) N. Melamed, et al., J. Neurosci. 13, 632 (1993) , (6) C. Du, et al., Biophys. J. 80, 549 (2001) , (7) Y.N. Tallini, et al., PNAS 103, 4753 (2006) , (8) P.R. Territo, et al., Appl. Spectrosc. 61, 138 (2007) , (9) B.R. Choi, Methods Mol. Biol. 937, 217 (2013)
- InChi
- InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52,/h10-17,20-23H,18-19,24-31H2,1-9H3,1H/q+1,/p-1
Long-wavelength cell-permeant Ca2+ indicator. Rhod-2/AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells via incubation and used for quantifying intracellular Ca2+ concentration allowing non-invasive measurement of calcium ions in live cells. This fluoregenic probe for intracellular Ca2+ operates in the visible spectrum. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-2 by endogenous esterases once the dye enters cells. Rhod-2 is a valuable alternative to Fluo-3 to investigate changes in intracellular free Ca2+ concentrations, especially in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Its signal intensity is the strongest of all the calcium probes. Spectral Data: ?ex 550nm, ?em 578nm in methanol, λex 549nm, λem 578nm in DMSO, λex 550nm, λem 570nm in Chloroform, Methanol (90:10)
Additional information
| Synonyms | 129787-64-0 |
|---|---|
| Purity | ≥80% (HPLC) |
| Appearance | Red-purple solid |
| CAS-Number | 145037-81-6 |
| Molecular Formula | C52H59BrN4O19 |
| Molecular Weight | 1123.96 |
| Solvents | DMSO, DMF, methanol, chloroform |
| Smiles | O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=C(C=C(C=C1)C2=C3C=C/C(C=C3OC4=CC(N(C)C)=CC=C24)=[N+](C)/C)OCCOC5=C(C=CC(C)=C5)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O.[Br-] |
| InChi Key | RWXWZOWDWYQKBK-UHFFFAOYSA-M |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) A. Minta, et al., J. Biol. Chem. 264, 8171 (1989), (2) T.M. Quinton, et al., BBRC 184, 893 (1992), (3) M. Okada, et al., Brain Res. 583, 227 (1992), (4) Y. Tamura, et al., Brain Res. 592, 317 (1992), (5) N. Melamed, et al., J. Neurosci. 13, 632 (1993), (6) C. Du, et al., Biophys. J. 80, 549 (2001), (7) Y.N. Tallini, et al., PNAS 103, 4753 (2006), (8) P.R. Territo, et al., Appl. Spectrosc. 61, 138 (2007), (9) B.R. Choi, Methods Mol. Biol. 937, 217 (2013) |
| InChi | InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52,/h10-17,20-23H,18-19,24-31H2,1-9H3,1H/q+1,/p-1 |
| Quantity | 1 mg, Bulk |
