Specifications
- Purity
- ≥80% (Dye content)
- Appearance
- Dark red to brown to green powder
- Impurities
- ≤0.5% crystal violet
Properties
- Solvents
- water (1 mg/ml), ethanol, methanol or DMSO.
Category: Non Fluorescent Detection & Chromogene Detection
Marker dye for DNA in acid and neutral media. Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. At high concentrations it is present at all available DNA sites. Methyl Green is used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. Spectral data: Absorption maxima = 630-635nm. May be used as a pH indicator with a pH range = pH 0.2(yellow) to pH 1.8(blue).
Synonyms | Ethyl Green, CI 42590 |
---|---|
Purity | ≥80% (Dye content) |
Appearance | Dark red to brown to green powder |
CAS-Number | 7114-03-6 |
Molecular Formula | C27H35BrClN3 · ZnCl2 |
Molecular Weight | 653.24 |
Solvents | water (1 mg/ml), ethanol, methanol or DMSO. |
Smiles | Cl[Zn]Cl.CN(C)C1=CC=C(/C(C2=CC=C([N+](C)(CC)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Br-].[Cl-] |
InChi Key | RRKSQWOQZFIQKW-UHFFFAOYSA-J |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
Hazard statements | H314 – H411 |
Precautionary statements | P273 – P280 – P305 + P351 + P338 + P310 – P391 – P501 |
GHS Symbol | GHS05+GHS09 |
Signal word | Danger |
Transportation | Not dangerous goods |
References | (1) H. Lyon, et al., Histochem. J. 24, 224 (1992), (2) S.K. Kim & B. Norden, FEBS Lett. 315, 61-64 (1993), (3) R.W. Sabnis, Handbook of Acid-Base indicators (2008), (4) D. Prieto, et al., Histochem. Cell Biol. 142, 335 (2014), (5) P.Murgai, et al., Anal. Biochem. 601, 113782 (2020) |
InChi | InChI=1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4 |
Quantity | 5 g, 25 g, Bulk |
cdx-Impurities | ≤0.5% crystal violet |