Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow-orange solid
- Identity
- 1H-NMR
Properties
- Solvents
- water, DMSO, DMF
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
Downloads
- Safety Data Sheet
- CDX C0014 MSDS.pdf
Available from stock
5-FAM
- SKU
- C0014
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-Carboxyfluorescein
- 76823-03-5
- CAS-Number
- C21H12O7
- Molecular Formula
- 376.32
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
- Smiles
- OC(=O)C1=CC=C(C(=C1)C(O)=O)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
- InChi Key
- VPMWLNJETPKSSC-UHFFFAOYSA-N
- References
- (1) R. Fischer et al. Bioconjugate Chem. 14, 653 (2003) , (2) E. Ralston et al. Biochim. Biophys. Acta, Biomembranes 649(1), 133 (1981)
- InChi
- InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)13-4-1-10(20(24)25)7-16(13)21(26)27/h1-9,22H,(H,24,25)(H,26,27)
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
Additional information
| Synonyms | 5-Carboxyfluorescein |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Yellow-orange solid |
| CAS-Number | 76823-03-5 |
| Molecular Formula | C21H12O7 |
| Molecular Weight | 376.32 |
| Identity | 1H-NMR |
| Solvents | water, DMSO, DMF |
| Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 |
| Smiles | OC(=O)C1=CC=C(C(=C1)C(O)=O)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| InChi Key | VPMWLNJETPKSSC-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) R. Fischer et al. Bioconjugate Chem. 14, 653 (2003), (2) E. Ralston et al. Biochim. Biophys. Acta, Biomembranes 649(1), 133 (1981) |
| InChi | InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)13-4-1-10(20(24)25)7-16(13)21(26)27/h1-9,22H,(H,24,25)(H,26,27) |
| Quantity | 25 mg, 100 mg, 1 g, Bulk |
