Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Green powder
Properties
- Fluorescence
- λex 575 nm, λem 600 nm
- Solvents
- methanol, DMSO, DMF
Available from stock
5-ROX
- SKU
- C0004
Category: Fluorescent Detection
- Synonyms
- 5-Carboxy-X-rhodamine , 5-CXR
- 216699-35-3
- CAS-Number
- C33H30N2O5
- Molecular Formula
- 534.6
- Molecular Weight
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Long Term Storage
- +4°C
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P261, P305 + P351 + P338
- Hazard statements
- H315, H319, H335
- Description
- 5-ROX is the purified isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is really critical. The minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. Used for oligonucleotide labeling and automated DNA sequencing applications.
- InChi
- InChI=1S/C33H30N2O5/c36-32(37)20-9-10-21(24(17-20)33(38)39)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39)
- References
- (1) P.J. Finn et al.: Nucleic Acids Res. 30, 2877 (2002) , (2) J.M. Bartlett et al.: Mol Biotechnol 13, 185 (1999) , (3) S.C. Hung et al.: Anal Biochem 255, 32 (1998)
- InChi Key
- UNGMOMJDNDFGJG-UHFFFAOYSA-N
- Smiles
- OC(=O)C1=CC=C(C(=C1)C([O-])=O)C1=C2C=C3CCC[N+]4=C3C(CCC4)=C2OC2=C3CCCN4CCCC(C=C12)=C34
5-ROX is the purified isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is really critical. The minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. Used for oligonucleotide labeling and automated DNA sequencing applications.
Additional information
| Synonyms | 5-Carboxy-X-rhodamine, 5-CXR |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Green powder |
| CAS-Number | 216699-35-3 |
| Molecular Formula | C33H30N2O5 |
| Molecular Weight | 534.6 |
| Identity | 1H-NMR |
| Solvents | methanol, DMSO, DMF |
| Fluorescence | λex 575 nm, λem 600 nm |
| Smiles | OC(=O)C1=CC=C(C(=C1)C([O-])=O)C1=C2C=C3CCC[N+]4=C3C(CCC4)=C2OC2=C3CCCN4CCCC(C=C12)=C34 |
| InChi Key | UNGMOMJDNDFGJG-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) P.J. Finn et al.: Nucleic Acids Res. 30, 2877 (2002), (2) J.M. Bartlett et al.: Mol Biotechnol 13, 185 (1999), (3) S.C. Hung et al.: Anal Biochem 255, 32 (1998) |
| InChi | InChI=1S/C33H30N2O5/c36-32(37)20-9-10-21(24(17-20)33(38)39)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39) |
| Quantity | 20 mg, 200 mg, Bulk |
