Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow to orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMF, DMSO, dimethylformamide
- Melting Point
- 223-227 °C (dec.)
- Fluorescence
- λex 492 nm, λem 517 nm in DMF
Available from stock
5(6)-FAM N-succinimidyl ester
- SKU
- C0011
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5(6)-Carboxyfluorescein NHS ester (mixture of isomers) , 5(6)-CFSE , 5(6)-FAM SE , FLUOS
- 117548-22-8
- CAS-Number
- C25H15NO9
- Molecular Formula
- 473.39
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: ?ex= 492| ?em=517nm in DMF or 0.1 M Tris, pH 8.0.
- Smiles
- OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O
- InChi Key
- STUDUJDWFUAOEN-UHFFFAOYSA-N
- References
- (1) P.R. Banks & D.M. Paquette , Bioconjug. Chem. 6, 447 (1995) , (2) P. Breeuwer, et al. , Appl. Environ. Microbiol. 62, 178 (1996) , (3) D. Fulcher & S. Wong , Immunol. Cell Biol. 77, 559 (1999) , (4) T. Sathiyaseelan & C.L. Baldwin , Res. Vet. Sci. 69, 275 (2000) , (5) I. Tsuge, et al. , Allergol. Int. 56, 149 (2007)
- InChi
- InChI=1S/C20H12O5.C6H7NO4/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24 , 1-4(8)11-7-5(9)2-3-6(7)10/h1-10,21H,(H,23,24) , 2-3H2,1H3
Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: ?ex= 492| ?em=517nm in DMF or 0.1 M Tris, pH 8.0.
Additional information
| Synonyms | 5(6)-Carboxyfluorescein NHS ester (mixture of isomers), 5(6)-CFSE, 5(6)-FAM SE, FLUOS |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow to orange powder |
| CAS-Number | 117548-22-8 |
| Molecular Formula | C25H15NO9 |
| Molecular Weight | 473.39 |
| Identity | 1H-NMR |
| Solvents | DMF, DMSO, dimethylformamide |
| Melting Point | 223-227 °C (dec.) |
| Fluorescence | λex 492 nm, λem 517 nm in DMF |
| Smiles | OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O |
| InChi Key | STUDUJDWFUAOEN-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) P.R. Banks & D.M. Paquette, Bioconjug. Chem. 6, 447 (1995), (2) P. Breeuwer, et al., Appl. Environ. Microbiol. 62, 178 (1996), (3) D. Fulcher & S. Wong, Immunol. Cell Biol. 77, 559 (1999), (4) T. Sathiyaseelan & C.L. Baldwin, Res. Vet. Sci. 69, 275 (2000), (5) I. Tsuge, et al., Allergol. Int. 56, 149 (2007) |
| InChi | InChI=1S/C20H12O5.C6H7NO4/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24, 1-4(8)11-7-5(9)2-3-6(7)10/h1-10,21H,(H,23,24), 2-3H2,1H3 |
| Quantity | 100 mg, Bulk |
