- ≥98% (HPLC)
- Yellow to orange powder
- λex 492 nm, λem 517 nm in DMF
- Melting Point
- 223-227 °C (dec.)
- DMF, DMSO, dimethylformamide
Category: Fluorescent Detection
Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: ?ex= 492| ?em=517nm in DMF or 0.1 M Tris, pH 8.0.
5(6)-Carboxyfluorescein NHS ester (mixture of isomers), 5(6)-CFSE, 5(6)-FAM SE, FLUOS
Yellow to orange powder
DMF, DMSO, dimethylformamide
223-227 °C (dec.)
λex 492 nm, λem 517 nm in DMF
|Short Term Storage|
|Long Term Storage|
Protect from light and moisture.
|Use / Stability|
Stable for at least 2 years after receipt when stored at -20°C.
Not dangerous goods
(1) P.R. Banks & D.M. Paquette, Bioconjug. Chem. 6, 447 (1995), (2) P. Breeuwer, et al., Appl. Environ. Microbiol. 62, 178 (1996), (3) D. Fulcher & S. Wong, Immunol. Cell Biol. 77, 559 (1999), (4) T. Sathiyaseelan & C.L. Baldwin, Res. Vet. Sci. 69, 275 (2000), (5) I. Tsuge, et al., Allergol. Int. 56, 149 (2007)
InChI=1S/C20H12O5.C6H7NO4/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24, 1-4(8)11-7-5(9)2-3-6(7)10/h1-10,21H,(H,23,24), 2-3H2,1H3
100 mg, Bulk