6-FAM N-succinimidyl ester

SKU: C0017

Category: Fluorescent Detection

Synonyms: 6-Carboxyfluorescein NHS ester , 6-CFSE , 6-FAM, SE; 92557-81-8
CAS-Number: C₂₅H₁₅NO₉
Molecular Formula: 473.39
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Yellow to orange powder

Properties

Fluorescence: λex 492 nm , λem 517 nm (pH 8.0)
Density: 1.74 g/cm3 (Predicted)
Refractive Index: n20/D 1.79 (Predicted)
Boiling Point: 788.09 °C at 760 mmHg (Predicted)
Melting Point: 337.43 °C (Predicted)
Solvents: DMSO, dimethylformamide

Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).
InChi Key: VDABVNMGKGUPEY-UHFFFAOYSA-N
InChi: InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
References: (1) P. Breeuwer, et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (2) M. Adamczyk, et al.: Bioconjugate Chem. 8, 253 (1997) , (3) T.S. Seo, et al.: J. Org. Chem. 68, 609 (2003) , (4) K. Tanaka, et al.: JACS 129, 5612 (2007) , (5) G.V. Los, et al.: ACS Chem. Biol. 3, 373 (2008) , (6) S. Mizukami, et al.: JACS 134, 1623 (2012)
Smiles: OC1=CC=C2C(OC(C=C(O)C=C3)=C3C24OC(C5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)=O)=C1

Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).

SKU: C0017 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	6-Carboxyfluorescein NHS ester, 6-CFSE, 6-FAM, SE
Purity	≥98% (HPLC)
Appearance	Yellow to orange powder
CAS-Number	92557-81-8
Molecular Formula	C25H15NO9
Molecular Weight	473.39
Identity	1H-NMR
Solvents	DMSO, dimethylformamide
Melting Point	337.43 °C (Predicted)
Boiling Point	788.09 °C at 760 mmHg (Predicted)
Refractive Index	n20/D 1.79 (Predicted)
Density	1.74 g/cm3 (Predicted)
Fluorescence	λex 492 nm, λem 517 nm (pH 8.0)
Smiles	OC1=CC=C2C(OC(C=C(O)C=C3)=C3C24OC(C5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)=O)=C1
InChi Key	VDABVNMGKGUPEY-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) P. Breeuwer, et al.: Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.: Bioconjugate Chem. 8, 253 (1997), (3) T.S. Seo, et al.: J. Org. Chem. 68, 609 (2003), (4) K. Tanaka, et al.: JACS 129, 5612 (2007), (5) G.V. Los, et al.: ACS Chem. Biol. 3, 373 (2008), (6) S. Mizukami, et al.: JACS 134, 1623 (2012)
InChi	InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
Quantity	10 mg, 25 mg, 100 mg, Bulk