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    6-FAM N-succinimidyl ester

    Available from stock

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      6-FAM N-succinimidyl ester

      SKU
      C0017

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      6-Carboxyfluorescein NHS ester , 6-CFSE , 6-FAM, SE
      92557-81-8
      CAS-Number
      C25H15NO9
      Molecular Formula
      473.39
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (HPLC)
      Appearance
      Yellow to orange powder
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO, dimethylformamide
      Melting Point
      337.43 °C (Predicted)
      Boiling Point
      788.09 °C at 760 mmHg (Predicted)
      Refractive Index
      n20/D 1.79 (Predicted)
      Density
      1.74 g/cm3 (Predicted)
      Fluorescence
      λex 492 nm , λem 517 nm (pH 8.0)
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Transportation
      Not dangerous goods
      Description
      Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).
      Smiles
      OC1=CC=C2C(OC(C=C(O)C=C3)=C3C24OC(C5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)=O)=C1
      InChi Key
      VDABVNMGKGUPEY-UHFFFAOYSA-N
      References
      (1) P. Breeuwer, et al.: Appl. Environ. Microbiol. 62, 178 (1996) , (2) M. Adamczyk, et al.: Bioconjugate Chem. 8, 253 (1997) , (3) T.S. Seo, et al.: J. Org. Chem. 68, 609 (2003) , (4) K. Tanaka, et al.: JACS 129, 5612 (2007) , (5) G.V. Los, et al.: ACS Chem. Biol. 3, 373 (2008) , (6) S. Mizukami, et al.: JACS 134, 1623 (2012)
      InChi
      InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2

      Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: ?ex 496 nm| ?em 516 nm (Buffer pH 9.0).

      SKU: C0017 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      6-Carboxyfluorescein NHS ester, 6-CFSE, 6-FAM, SE

      Purity

      ≥98% (HPLC)

      Appearance

      Yellow to orange powder

      CAS-Number

      92557-81-8

      Molecular Formula

      C25H15NO9

      Molecular Weight

      473.39

      Identity

      1H-NMR

      Solvents

      DMSO, dimethylformamide

      Melting Point

      337.43 °C (Predicted)

      Boiling Point

      788.09 °C at 760 mmHg (Predicted)

      Refractive Index

      n20/D 1.79 (Predicted)

      Density

      1.74 g/cm3 (Predicted)

      Fluorescence

      λex 492 nm, λem 517 nm (pH 8.0)

      Smiles

      OC1=CC=C2C(OC(C=C(O)C=C3)=C3C24OC(C5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)=O)=C1

      InChi Key

      VDABVNMGKGUPEY-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) P. Breeuwer, et al.: Appl. Environ. Microbiol. 62, 178 (1996), (2) M. Adamczyk, et al.: Bioconjugate Chem. 8, 253 (1997), (3) T.S. Seo, et al.: J. Org. Chem. 68, 609 (2003), (4) K. Tanaka, et al.: JACS 129, 5612 (2007), (5) G.V. Los, et al.: ACS Chem. Biol. 3, 373 (2008), (6) S. Mizukami, et al.: JACS 134, 1623 (2012)

      InChi

      InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2

      Quantity

      10 mg, 25 mg, 100 mg, Bulk

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