Available from stock

6-FITC

SKU: F0111

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: Fluorescein 6-isothiocyanate , Fluorescein-4-isothiocyanate , 2-(3-Hydroxy-6-oxoxanthen-9-yl)-4-isothiocyanatobenzoic acid , FITC Isomer II , FITC-II; 18861-78-4
CAS-Number: C₂₁H₁₁NO₅S
Molecular Formula: 389.39
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Appearance: Yellow to brown powder
Identity: 1H-NMR

Properties

Solvents: ethanol, DMSO, DMF

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H302, H315, H317, H319, H332, H334 , H335
Precautionary statements: P261, P264, P270-P272, P280, P285, P363, P301+P312, P302+P352, P304+P340, P305+P351+P338, P342+P311, P403+P233, P405, P501
GHS Symbol: GHS07+GHS08
Signal word: Danger
Transportation: Not dangerous goods

Description: 6-Fluorescein isothiocyanate (6-FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Spectral data: ?ex=492nm, ?em=518nm.
Smiles: O=C(C1=C2C=C(N=C=S)C=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53
InChi Key: GTQFZXYECNSNNC-UHFFFAOYSA-N
References: (1) T.J. Porro, et al. , Stain Technol. 38, 37 (1963) , (2) H.S. Corey & R.M. McKinney , Anal. Biochem. 10, 387 (1965) , (3) R.M. McKinney, et al. , Anal. Biochem. 29, 526 (1969) , (4) J. Sinsheimer, et al. , Anal. Biochem. 57, 227 (1974) , (5) D.L. Reynolds & L.A. Pachla , J. Pharm. Sci. 74, 1091 (1985) , (6) R. Zuckermann, et al. , Nucl. Acids Res. 15, 5305 (1987) , (7) C.A. Swindlehurst & E.W. Voss , Biophys. J. 59, 619 (1991) , (8) C. Wang, et al. , BBRC 331, 351 (2005)

6-Fluorescein isothiocyanate (6-FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Spectral data: ?ex=492nm, ?em=518nm.

SKU: F0111 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	Fluorescein 6-isothiocyanate, Fluorescein-4-isothiocyanate, 2-(3-Hydroxy-6-oxoxanthen-9-yl)-4-isothiocyanatobenzoic acid, FITC Isomer II, FITC-II
Purity	≥90% (HPLC)
Appearance	Yellow to brown powder
CAS-Number	18861-78-4
Molecular Formula	C21H11NO5S
Molecular Weight	389.39
Identity	1H-NMR
Solvents	ethanol, DMSO, DMF
Smiles	O=C(C1=C2C=C(N=C=S)C=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53
InChi Key	GTQFZXYECNSNNC-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H302, H315, H317, H319, H332, H334 , H335
Precautionary statements	P261, P264, P270-P272, P280, P285, P363, P301+P312, P302+P352, P304+P340, P305+P351+P338, P342+P311, P403+P233, P405, P501
GHS Symbol	GHS07+GHS08
Signal word	Danger
Transportation	Not dangerous goods
References	(1) T.J. Porro, et al., Stain Technol. 38, 37 (1963), (2) H.S. Corey & R.M. McKinney, Anal. Biochem. 10, 387 (1965), (3) R.M. McKinney, et al., Anal. Biochem. 29, 526 (1969), (4) J. Sinsheimer, et al., Anal. Biochem. 57, 227 (1974), (5) D.L. Reynolds & L.A. Pachla, J. Pharm. Sci. 74, 1091 (1985), (6) R. Zuckermann, et al., Nucl. Acids Res. 15, 5305 (1987), (7) C.A. Swindlehurst & E.W. Voss, Biophys. J. 59, 619 (1991), (8) C. Wang, et al., BBRC 331, 351 (2005)
Quantity	100 mg, 250 mg, Bulk