Specifications
- Purity
- for microscopy
- Appearance
- Colourless liquid
- Identity
- 1H-NMR
Properties
- Refractive Index
- n20/D 1.361
- Density
- 0.80 g/ml at 20 °C
Downloads
- Safety Data Sheet
- CDX G0062 MSDS.pdf
Category: Non Fluorescent Detection & Chromogene Detection
Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Purity | for microscopy |
---|---|
Appearance | Colourless liquid |
CAS-Number | 37348-17-7 (comp.) |
Molecular Formula | n/a |
Molecular Weight | n/a |
Identity | 1H-NMR |
Refractive Index | n20/D 1.361 |
Density | 0.80 g/ml at 20 °C |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | This product should not be used if colour has changed or if there are |
Hazard statements | H225-H319-H336 |
Precautionary statements | P210-P280-P304 + P340 + P312-P305 + P351 + P338-P337 + P313-P403 + P235 |
GHS Symbol | GHS02+GHS07 |
Signal word | Danger |
RIDADR | UN 1993BF |
Transportation | Packing Group II |
References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
Quantity | 250 ml, 2.5 l, Bulk |