Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow to red solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or dimethylformamide
- Fluorescence
- λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0
Documentation
- Safety Data Sheet (SDS)
- CDX C0002 MSDS_V1.1.pdf
Available from stock
BCECF acid
- SKU
- C0002
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein
- 85138-49-4
- CAS-Number
- C27H20O11
- Molecular Formula
- 520.4
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
- Smiles
- OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(O)=O)C=C1O.OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=C(C=C3)C(O)=O)C=C1O
- InChi Key
- RZFSVSQVNQXHLI-UHFFFAOYSA-N
- References
- (1) L. Homolya, et al.: J. Biol. Chem. 268, 21493 (1993) , (2) H. Kao, et al.: J. Cell Biol. 120, 175 (1993) , (3) K. Fushimi, et al.: J. Cell Biol. 112, 719 (1991) , (4) H. Szmacinski, et al.: Analyt. Chem. 65, 1668 (1993)
- InChi
- InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21
Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
Additional information
| Synonyms | 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Yellow to red solid |
| CAS-Number | 85138-49-4 |
| Molecular Formula | C27H20O11 |
| Molecular Weight | 520.4 |
| Identity | 1H-NMR |
| Solvents | DMSO or dimethylformamide |
| Fluorescence | λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0 |
| Smiles | OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(O)=O)C=C1O.OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=C(C=C3)C(O)=O)C=C1O |
| InChi Key | RZFSVSQVNQXHLI-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) L. Homolya, et al.: J. Biol. Chem. 268, 21493 (1993), (2) H. Kao, et al.: J. Cell Biol. 120, 175 (1993), (3) K. Fushimi, et al.: J. Cell Biol. 112, 719 (1991), (4) H. Szmacinski, et al.: Analyt. Chem. 65, 1668 (1993) |
| InChi | InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21 |
| Quantity | 1 mg, 5 mg, 25 mg, Bulk |
