Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Yellow to red solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or dimethylformamide
- Fluorescence
- λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0
Documentation
- Safety Data Sheet (SDS)
- CDX C0002 MSDS_V1.1.pdf
Categories: Fluorescent Detection, Fluorescent Detection
Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
Synonyms | 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein |
---|---|
Purity | ≥95% (HPLC) |
Appearance | Yellow to red solid |
CAS-Number | 85138-49-4 |
Molecular Formula | C27H20O11 |
Molecular Weight | 520.4 |
Identity | 1H-NMR |
Solvents | DMSO or dimethylformamide |
Fluorescence | λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0 |
Smiles | OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(O)=O)C=C1O.OC(=O)CCC1=CC2=C(OC3=CC(O)=C(CCC(O)=O)C=C3C22OC(=O)C3=C2C=C(C=C3)C(O)=O)C=C1O |
InChi Key | RZFSVSQVNQXHLI-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) L. Homolya, et al.: J. Biol. Chem. 268, 21493 (1993), (2) H. Kao, et al.: J. Cell Biol. 120, 175 (1993), (3) K. Fushimi, et al.: J. Cell Biol. 112, 719 (1991), (4) H. Szmacinski, et al.: Analyt. Chem. 65, 1668 (1993) |
InChi | InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21 |
Quantity | 1 mg, 5 mg, 25 mg, Bulk |