Available from stock

D-Biotin

SKU: B0148

Category: Vitamins

Synonyms: Bios II , Biotin , Coenzyme R , Vitamin B7 , Vitamin H , NSC 63865 , Factor S; 58-85-5
CAS-Number: C₁₀H₁₆N₂O₃S
Molecular Formula: 244.3
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (NMR)
Appearance: White to off-white powder
Identity: 1H-NMR

Properties

Solvents: DMSO (49 mg/ml at 25°C) and aqueous basic salt buffer (5 mg/ml)
Melting Point: 233-236 °C
Boiling Point: ~573.6 °C at 760 mmHg (Predicted)
Optical Activity: α20/D +91º±2º, c = 2 in 0.1N NaOH
Refractive Index: n20D 1.55 (Predicted)
Density: ~1.3 g/cm3 (Predicted)

Documentation

Safety Data Sheet (SDS): CDX B0148 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: D-(+)-Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). D-(+)-Biotin is a cofactor responsible for carbon dioxide transfer in five carboxylase enzymes, such as acetyl-CoA carboxylase alpha, acetyl-CoA carboxylase beta, methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase or pyruvate carboxylase. It is important in fatty acid synthesis, branched-chain amino acid catabolism and gluconeogenesis. It covalently attaches to the epsilon-amino group of specific lysine residues in these carboxylases. This biotinylation reaction requires ATP and is catalyzed by holocarboxylase synthetase. Biotin is also covalently attached to distinct lysine residues in histones, affecting chromatin structure and mediating gene regulation. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. The attachment of biotin to various chemical sites is used to study various processes, including protein localization, protein interactions, DNA transcription and replication.
Smiles: OC(CCCC[C@H]1[C@](N2)([H])[C@](NC2=O)([H])CS1)=O
InChi Key: YBJHBAHKTGYVGT-ZKWXMUAHSA-N
References: (1) A. Chapman-Smith & J.E. Cronan Jr., J. Nutr. 129, 477S (1999) , (2) J. Zempleni & D.M. Mock, J. Nutr. Biochem. 10, 128 (1999) , (3) A. Holmberg, et al., Electrophoresis 26, 501 (2005) , (4) J. Zempleni, et al., Biofactors 35, 36 (2009)
InChi: InChI=1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

D-(+)-Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). D-(+)-Biotin is a cofactor responsible for carbon dioxide transfer in five carboxylase enzymes, such as acetyl-CoA carboxylase alpha, acetyl-CoA carboxylase beta, methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase or pyruvate carboxylase. It is important in fatty acid synthesis, branched-chain amino acid catabolism and gluconeogenesis. It covalently attaches to the epsilon-amino group of specific lysine residues in these carboxylases. This biotinylation reaction requires ATP and is catalyzed by holocarboxylase synthetase. Biotin is also covalently attached to distinct lysine residues in histones, affecting chromatin structure and mediating gene regulation. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin’s small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. The attachment of biotin to various chemical sites is used to study various processes, including protein localization, protein interactions, DNA transcription and replication.

SKU: B0148 Category: Vitamins

Description
Additional information

Description

Additional information

Synonyms	Bios II, Biotin, Coenzyme R, Vitamin B7, Vitamin H, NSC 63865, Factor S
Purity	≥98% (NMR)
Appearance	White to off-white powder
CAS-Number	58-85-5
Molecular Formula	C10H16N2O3S
Molecular Weight	244.3
Identity	1H-NMR
Solvents	DMSO (49 mg/ml at 25°C) and aqueous basic salt buffer (5 mg/ml)
Melting Point	233-236 °C
Boiling Point	~573.6 °C at 760 mmHg (Predicted)
Optical Activity	α20/D +91º±2º, c = 2 in 0.1N NaOH
Refractive Index	n20D 1.55 (Predicted)
Density	~1.3 g/cm3 (Predicted)
Smiles	OC(CCCC[C@H]1[C@](N2)([H])[C@](NC2=O)([H])CS1)=O
InChi Key	YBJHBAHKTGYVGT-ZKWXMUAHSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) A. Chapman-Smith & J.E. Cronan Jr., J. Nutr. 129, 477S (1999), (2) J. Zempleni & D.M. Mock, J. Nutr. Biochem. 10, 128 (1999), (3) A. Holmberg, et al., Electrophoresis 26, 501 (2005), (4) J. Zempleni, et al., Biofactors 35, 36 (2009)
InChi	InChI=1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
Quantity	1 g, 5 g, 25 g, Bulk