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    DTNB

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      DTNB

      SKU
      D0156

      Category: Non Fluorescent Detection & Chromogene Detection

      Synonyms
      Ellman's Reagent , 5,5'-Dithiobis(2-nitrobenzoic acid) , 3-Carboxy-4-nitrophenyl disulfide , 6,6'-Dinitro-3,3'-dithiodibenzoic acid , Bis(3-carboxy-4-nitrophenyl) disulfide
      69-78-3
      CAS-Number
      C14H8N2O8S2
      Molecular Formula
      396.35
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (Titration)
      Identity
      1H-NMR
      Appearance
      Yellow powder

      Properties

      Melting Point
      240-245 °C
      Solvents
      ethanol, dioxane, phosphate buffer

      Downloads

      Safety Data Sheet
      CDX D0156 MSDS.pdf
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at RT.
      Long Term Storage
      RT
      Short Term Storage
      RT
      Shipping
      AMBIENT
      Transportation
      Packing Group III
      Signal word
      Warning
      GHS Symbol
      GHS07
      Precautionary statements
      P261-P305 + P351 + P338
      Hazard statements
      H315-H319-H335
      Description
      A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.
      InChi
      InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
      References
      (1) G.L. Ellman: Arch. Biochem. Biophys. 82, 70 (1959) , (2) H.B. Collier: Anal. Biochem. 56, 310 (1973) , (3) P.W. Riddles, et al.: Meth. Enzymol. 91, 49 (1983) , (4) C.K. Riener, et al.: Anal. Bioanal. Chem. 373, 266 (2002) , (5) J. Sedlak & R.H. Lindsay: Anal. Biochem. 25, 192 (1968) , (6) G.L. Ellman: Biochem. Pharmacol. 7, 88 (1961) , (7) M.J. Gething & B.E. Davidson: Eur. J. Biochem. 30, 352 (1972) , (8) A.F.S.A. Habeeb: Meth. Enzymol. 25, 457 (1972) , (9) D. Ming, et al.: Biotechniques 18, 808 (1995)
      InChi Key
      KIUMMUBSPKGMOY-UHFFFAOYSA-N
      Smiles
      O=[N+]([O-])C1=CC=C(SSC2=CC=C([N+]([O-])=O)C(C(O)=O)=C2)C=C1C(O)=O

      A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

      SKU: D0156 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Synonyms

      Ellman's Reagent, 5,5'-Dithiobis(2-nitrobenzoic acid), 3-Carboxy-4-nitrophenyl disulfide, 6,6'-Dinitro-3,3'-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide

      Purity

      ≥98% (Titration)

      Appearance

      Yellow powder

      CAS-Number

      69-78-3

      Molecular Formula

      C14H8N2O8S2

      Molecular Weight

      396.35

      Identity

      1H-NMR

      Solvents

      ethanol, dioxane, phosphate buffer

      Melting Point

      240-245 °C

      Smiles

      O=[N+]([O-])C1=CC=C(SSC2=CC=C([N+]([O-])=O)C(C(O)=O)=C2)C=C1C(O)=O

      InChi Key

      KIUMMUBSPKGMOY-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      RT

      Long Term Storage

      RT

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at RT.

      Hazard statements

      H315-H319-H335

      Precautionary statements

      P261-P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Packing Group III

      References

      (1) G.L. Ellman: Arch. Biochem. Biophys. 82, 70 (1959), (2) H.B. Collier: Anal. Biochem. 56, 310 (1973), (3) P.W. Riddles, et al.: Meth. Enzymol. 91, 49 (1983), (4) C.K. Riener, et al.: Anal. Bioanal. Chem. 373, 266 (2002), (5) J. Sedlak & R.H. Lindsay: Anal. Biochem. 25, 192 (1968), (6) G.L. Ellman: Biochem. Pharmacol. 7, 88 (1961), (7) M.J. Gething & B.E. Davidson: Eur. J. Biochem. 30, 352 (1972), (8) A.F.S.A. Habeeb: Meth. Enzymol. 25, 457 (1972), (9) D. Ming, et al.: Biotechniques 18, 808 (1995)

      InChi

      InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

      Quantity

      1 g, 5 g, 25 g, Bulk

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