Specifications
- Purity
- ≥97% (NMR)
- Appearance
- Pale beige to yellow solid
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in ethanol, dichloromethane or chloroform.
- Melting Point
- 54 °C
Category: Fluorescent Detection
Fluorescein didecanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the didecanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells. Fluorescein didecanoate is a fluorogenic substrate for C10 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: λEx 497nM, λEm 516nm. Extinction coefficient: 80000 cm-1 M-1.
Purity | ≥97% (NMR) |
---|---|
Appearance | Pale beige to yellow solid |
CAS-Number | 7389-09-5 |
Molecular Formula | C40H48O7 |
Molecular Weight | 640.80 |
Identity | 1H-NMR |
Solvents | Soluble in ethanol, dichloromethane or chloroform. |
Melting Point | 54 °C |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) B. Rotman & B.W. Papermaster, PNAS 55, 134 (1966), (2) F.-Y. Ge, et al., Dyes Pigm. 72, 322 (2007) |
Quantity | 100 mg, 250 mg, 1 g, Bulk |