Specifications
- Purity
- ≥97% (NMR)
- Appearance
- Off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- ethanol, chloroform
- Melting Point
- 161 °C
Category: Fluorescent Detection, Fluorescent Detection
Fluorescein is the most important xanthene dye widely used for labeling and sensing biomolecules. It has a high extinction coefficient and fluorescence quantum yield in aqueous solution, and its excitation wavelength is in the visible range. However, fluorescein also has some obvious deficiencies. Particularly its hydrophilic nature limits its application to the detection of enzymes, therefore fluorescein esters are a useful alternative. Fluorescein esters, such as fluorescein dipropionate, are nonfluorescent, but they can be cleaved by intracellular enzymes to yield fluorescent products, such as fluorescein. The rate of change in the fluorescence of the solution, due to the production of fluorescein, is measured and correlated with enzyme activity. Therefore, fluorescein esters could be used as fluorometric determination probes of enzymes. Fluorescein dipropionate could also be used as a building block or intermediate in synthesis of fluorescent probes.
Synonyms | FDPr, 3?,6?-Bis(1-oxopropoxy)spiro[isobenzofuran-1(3H),9?-[9H]xanthen]-3-one |
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Purity | ≥97% (NMR) |
Appearance | Off-white powder |
CAS-Number | 7276-28-0 |
Molecular Formula | C26H20O7 |
Molecular Weight | 444.43 |
Identity | 1H-NMR |
Solvents | ethanol, chloroform |
Melting Point | 161 °C |
Smiles | O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(CC)=O)C=C4OC5=C3C=CC(OC(CC)=O)=C5 |
InChi Key | NXIABCNYUKDBQI-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H319 |
Precautionary statements | P264-P280-P305+P351+P338-P337+P313 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) B. Rotman & B.W. Papermaster, PNAS 55, 134 (1966), (2) F.-Y. Ge, et al., Dyes Pigments 72, 322 (2007), (3) M. Poznik & B. Koenig, Org. Biomol. Chem. 12, 3175 (2014) |
InChi | InChI=1S/C26H20O7/c1-3-23(27)30-15-9-11-19-21(13-15)32-22-14-16(31-24(28)4-2)10-12-20(22)26(19)18-8-6-5-7-17(18)25(29)33-26/h5-14H,3-4H2,1-2H3 |
Quantity | 1 g, Bulk |