- Synonyms: 4-Chloro-7-nitrobenzofurazan
- Purity: ≥98% (Titration)
- Appearance: Yellow powder
- CAS-Number: 10199-89-0
- Molecular Formula: C6H2ClN3O3
- Molecular Weight: 199.55
- Identity: 1H-NMR
- Solvents: DMF
- Melting Point: 97-99 °C (lit.)
- Fluorescence: λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
- Smiles: [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12
- InChi Key: IGHBXJSNZCFXNK-UHFFFAOYSA-N
Available from stock
NBD-Cl
SKU: C0010
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: +4°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
- Description: NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
- References: (1) N. Feller, et al., Br. J. Cancer. 72, 543 (1995), (10) G. Szakacs, et al., Pathol. Oncol. Res. 4, 251 (2016), (2) N. Feller, et al., FEBS Lett. 368 385 (1995), (3) F. Tiberghien & F. Loor, Anti-Cancer Drugs 7, 568 (1996), (4) P. Decherchi, et al., J. Neurosci. Methods 71, 205 (1997), (5) M. Essodaigui, et al., Biochemistry 37, 2243 (1998), (6) J.J. Lemasters, et al., Biochim. Biophys. Acta. 1366, 177 (1998), (7) J. Hynes, et al., J. Biomol. Screen. 8, 264 (2003), (8) D. Bratosin, et al., Cytometry A 66, 78 (2005), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010)
NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
Additional information
| Identity | 1H-NMR |
|---|---|
| Availability | 1 |
| CAS-Number | 10199-89-0 |
| Fluorescence | λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine) |
| InChi | |
| Melting Point | 97-99 °C (lit.) |
| Quantity | 1 g, 10 g, 50 g, Bulk |
| Appearance | Yellow powder |
| InChi Key | IGHBXJSNZCFXNK-UHFFFAOYSA-N |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) N. Feller, et al., Br. J. Cancer. 72, 543 (1995), (2) N. Feller, et al., FEBS Lett. 368 385 (1995), (3) F. Tiberghien & F. Loor, Anti-Cancer Drugs 7, 568 (1996), (4) P. Decherchi, et al., J. Neurosci. Methods 71, 205 (1997), (5) M. Essodaigui, et al., Biochemistry 37, 2243 (1998), (6) J.J. Lemasters, et al., Biochim. Biophys. Acta. 1366, 177 (1998), (7) J. Hynes, et al., J. Biomol. Screen. 8, 264 (2003), (8) D. Bratosin, et al., Cytometry A 66, 78 (2005), (9) R.W. Sabnis, Handbook of biological dyes and stains (2010), (10) G. Szakacs, et al., Pathol. Oncol. Res. 4, 251 (2016) |
| Smiles | [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12 |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Molecular Weight | |
| Solvents | DMF |
| Synonyms | 4-Chloro-7-nitrobenzofurazan |
| Shipping | AMBIENT |
| Top Sellers | Fluorescent Detection |
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