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    NBD-Cl

    Available from stock

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      NBD-Cl

      SKU
      C0010

      Category: Fluorescent Detection

      Synonyms
      4-Chloro-7-nitrobenzofurazan
      10199-89-0
      CAS-Number
      C6H2ClN3O3
      Molecular Formula
      199.55
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (GC)
      Identity
      1H-NMR
      Appearance
      Yellow powder

      Properties

      Fluorescence
      λex 420 nm, λem ~540 nm in ethanol (after derivatization with glycine)
      Melting Point
      97-99 °C (lit.)
      Solvents
      DMF, methanol, chloroform

      Downloads

      Safety Data Sheet
      CDX C0010 MSDS.pdf
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at +4°C.
      Long Term Storage
      +4°C
      Short Term Storage
      +4°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      RIDADR
      UN2811 6.1
      Signal word
      Warning
      GHS Symbol
      GHS07
      Precautionary statements
      P305 + P351 + P338
      Hazard statements
      H315-H319
      Description
      NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
      InChi
      InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
      References
      (1) Z. Kamenik et al.: Anal. Bioanal. Chem. 393(6-7), 1779 (2009) , (2) M. Maroulis et al.: J. Chrom. 876(2), 245 (2008) , (3) G. Ricci et al.: Anal. Biochem. 218, 463 (1994) , (4) L.-G. Martensson et al.: Biochem. 32, 224 (1993) , (5) A. Chattopadhyay et al.: Review. Chem. Phys. Lipids 53, 1 (1990) , (6) P.B. Ghosh et al.: J. Biochem. 108, 155 (1968)
      InChi Key
      IGHBXJSNZCFXNK-UHFFFAOYSA-N
      Smiles
      [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12

      NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

      SKU: C0010 Category: Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      4-Chloro-7-nitrobenzofurazan

      Purity

      ≥98% (GC)

      Appearance

      Yellow powder

      CAS-Number

      10199-89-0

      Molecular Formula

      C6H2ClN3O3

      Molecular Weight

      199.55

      Identity

      1H-NMR

      Solvents

      DMF, methanol, chloroform

      Melting Point

      97-99 °C (lit.)

      Fluorescence

      λex 420 nm, λem ~540 nm in ethanol (after derivatization with glycine)

      Smiles

      [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12

      InChi Key

      IGHBXJSNZCFXNK-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      +4°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at +4°C.

      Hazard statements

      H315-H319

      Precautionary statements

      P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      RIDADR

      UN2811 6.1

      Transportation

      Not dangerous goods

      References

      (1) Z. Kamenik et al.: Anal. Bioanal. Chem. 393(6-7), 1779 (2009), (2) M. Maroulis et al.: J. Chrom. 876(2), 245 (2008), (3) G. Ricci et al.: Anal. Biochem. 218, 463 (1994), (4) L.-G. Martensson et al.: Biochem. 32, 224 (1993), (5) A. Chattopadhyay et al.: Review. Chem. Phys. Lipids 53, 1 (1990), (6) P.B. Ghosh et al.: J. Biochem. 108, 155 (1968)

      InChi

      InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

      Quantity

      1 g, 10 g, 50 g, Bulk

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