NBD-Cl

SKU: C0010

Category: Fluorescent Detection

Synonyms: 4-Chloro-7-nitrobenzofurazan; 10199-89-0
CAS-Number: C₆H₂ClN₃O₃
Molecular Formula: 199.55
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (GC)
Identity: 1H-NMR
Appearance: Yellow powder

Properties

Fluorescence: λex 420 nm, λem ~540 nm in ethanol (after derivatization with glycine)
Melting Point: 97-99 °C (lit.)
Solvents: DMF, methanol, chloroform

Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Long Term Storage: +4°C
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
RIDADR: UN2811 6.1
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P305 + P351 + P338
Hazard statements: H315-H319

Description: NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
InChi: InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
References: (1) Z. Kamenik et al.: Anal. Bioanal. Chem. 393(6-7), 1779 (2009) , (2) M. Maroulis et al.: J. Chrom. 876(2), 245 (2008) , (3) G. Ricci et al.: Anal. Biochem. 218, 463 (1994) , (4) L.-G. Martensson et al.: Biochem. 32, 224 (1993) , (5) A. Chattopadhyay et al.: Review. Chem. Phys. Lipids 53, 1 (1990) , (6) P.B. Ghosh et al.: J. Biochem. 108, 155 (1968)
InChi Key: IGHBXJSNZCFXNK-UHFFFAOYSA-N
Smiles: [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12

NBD chloride is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

SKU: C0010 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	4-Chloro-7-nitrobenzofurazan
Purity	≥98% (GC)
Appearance	Yellow powder
CAS-Number	10199-89-0
Molecular Formula	C6H2ClN3O3
Molecular Weight	199.55
Identity	1H-NMR
Solvents	DMF, methanol, chloroform
Melting Point	97-99 °C (lit.)
Fluorescence	λex 420 nm, λem ~540 nm in ethanol (after derivatization with glycine)
Smiles	[O-][N+](=O)C1=CC=C(Cl)C2=NON=C12
InChi Key	IGHBXJSNZCFXNK-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H315-H319
Precautionary statements	P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
RIDADR	UN2811 6.1
Transportation	Not dangerous goods
References	(1) Z. Kamenik et al.: Anal. Bioanal. Chem. 393(6-7), 1779 (2009), (2) M. Maroulis et al.: J. Chrom. 876(2), 245 (2008), (3) G. Ricci et al.: Anal. Biochem. 218, 463 (1994), (4) L.-G. Martensson et al.: Biochem. 32, 224 (1993), (5) A. Chattopadhyay et al.: Review. Chem. Phys. Lipids 53, 1 (1990), (6) P.B. Ghosh et al.: J. Biochem. 108, 155 (1968)
InChi	InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
Quantity	1 g, 10 g, 50 g, Bulk