Specifications
- Purity
- ≥97% (TLC)
- Appearance
- Orange to brown solid
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO (20mg/ml).
- Fluorescence
- λex 571 nm, λem 590 nm in 0.1 M phosphate pH 7.0 (β-galactosidase)
Category: Fluorescent Detection, Fluorescent Detection
Resorufin &beta,-D-galactopyranoside is a fluorogenic substrate for &beta,-galactosidase. Upon enzymatic cleavage by the enzymes, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=584nm. It is used in several applications, specifically in compound screens, where it is advantageous because it is less prone to compound autofluorescence, and thus produces less false positives. It is widely used as substrate in high throughput screening of galactosidase inhibitors.
Synonyms | 3-Phenoxazone 7-(β-D-galactopyranoside) |
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Purity | ≥97% (TLC) |
Appearance | Orange to brown solid |
CAS-Number | 95079-19-9 |
Molecular Formula | C18H17NO8 |
Molecular Weight | 375.33 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO (20mg/ml). |
Fluorescence | λex 571 nm, λem 590 nm in 0.1 M phosphate pH 7.0 (β-galactosidase) |
Smiles | O=C1C=C2OC3=CC(O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)=CC=C3N=C2C=C1 |
InChi Key | QULZFZMEBOATFS-DISONHOPSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) J. Hofmann & M. Sernetz, Anal. Chim. Acta 163, 67 (1984), (2) K.D. Wittrup & J.E. Bailey, Cytometry 9, 394 (1988), (3) A.G. Hadd, et al., Anal. Chem. 69, 3407 (1997), (4) M.J. Eggertson & D.B. Craig, Biomed. Chromatogr. 13, 516 (1999), (5) G.K. Shoemaker, et al., Biochemistry 42, 1707 (2003), (6) S. Jambovane, et al., Anal. Chem. 81, 3239 (2009) |
Quantity | 10 mg, Bulk |