Specifications
- Appearance
- Greenish red powder
- Identity
- 1H-NMR
Properties
- Solvents
- methanol, water, ethanol
- Boiling Point
- <200 °C
- UV / Vis
- λmax 524 nm
Categories: Fluorescent Detection, Fluorescent Detection
Rhodamine 6G is a highly fluorescent hydrophilic pH-sensitive dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. The dye has a remarkably high photostability and high fluorescence quantum yield. Used as a laser dye and potential mitochondrial probe. The lasing range of the dye is 555 to 585 nm with a maximum at 566 nm. Useful in Pgp efflux assays and has been used to characterize kinetics of MRP1-mediated efflux. Spectral data: ?ex 528 nm| ?em 551 nm (in MeOH).
Synonyms | 6-Carboxyrhodamine, Aizen Rhodamine 6GCP, Basic Red 1, C.I. 45160, Calcozine Red 6G, NSC 36345 |
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Appearance | Greenish red powder |
CAS-Number | 989-38-8 |
Molecular Formula | C28H30N2O3 . HCl |
Molecular Weight | 479.02 |
Identity | 1H-NMR |
Solvents | methanol, water, ethanol |
Boiling Point | <200 °C |
UV / Vis | λmax 524 nm |
Smiles | CC1=CC2=C(C3=C(C(OCC)=O)C=CC=C3)C4=CC(C)=C(NCC)C=C4OC2=C/C1=NCC.Cl |
InChi Key | VYXSBFYARXAAKO-WTKGSRSZSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H302 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) T. Aiuchi, et al.: Biochim. Biophys. Acta. 685, 289 (1982), (2) R.F. Kubin & A.N. Fletcher, J. Luminesc. 27, 455 (1983), (3) M.W. Berns, et al.: Cell Biophys. 6, 263 (1984), (4) Y. Matsumoto, et al.: J. Neurooncol. 13, 217 (1992), (5) M. Mandala, et al.: Anal. Biochem. 274, 1 (1999), (6) D. Magde, et al.: Photochem. Photobiol. 75, 327 (2002), (7) Z. Chen, et al.: J. Fluoresc. 18, 93 (2008), (8) T. Baumgartel, et al.: Nanotechnology 21, 475205 (2010) |
InChi | InChI=1S/C28H30N2O3.ClH/c1-6-29-23-15-25-21(13-17(23)4)27(19-11-9-10-12-20(19)28(31)32-8-3)22-14-18(5)24(30-7-2)16-26(22)33-25 |
Quantity | 25 g, 100 g, Bulk |