Specifications
- Purity
- for microscopy
- Appearance
- Dark red liquid
- Identity
- UV/Vis
Properties
- Solvents
- water
- Refractive Index
- n20/D 1.334
- Density
- 0.999 g/ml at 20 °C
Available from stock
Gram’s fuchsin solution
- SKU
- G0064
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- Fuchsin solution , Basic Red 9 , Magenta O , Basic Violet 14 , 58969-01-0 (component)
- 632-99-5 (component)
- CAS-Number
- C20H20ClN3
- Molecular Formula
- 337.85
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- This product should not be used if colour has changed or if there are
- Hazard statements
- H350
- Precautionary statements
- P201-P308 + P313
- GHS Symbol
- GHS08
- Transportation
- Not dangerous goods
- Description
- Gram's Fuchsin Solution is a reagent that could be used in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue/violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by Gram's Fuchsin solution and appear red/pink. Both Gram-positive bacteria and Gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on Gram-positive bacteria because of the darker crystal violet stain.
- Smiles
- NC1=CC=C(/C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C/3)=N)C=C1.Cl
- InChi Key
- NIKFYOSELWJIOF-SVFFXJIWSA-N
- References
- (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) T.J. Beveridge, Biotech. Histochem. 76, 111 (2001) , (3) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)
- InChi
- InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15,/h2-12,21H,22-23H2,1H3,1H/b20-14-,21-17?,
Gram’s Fuchsin Solution is a reagent that could be used in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue/violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by Gram’s Fuchsin solution and appear red/pink. Both Gram-positive bacteria and Gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on Gram-positive bacteria because of the darker crystal violet stain.
Additional information
| Synonyms | Fuchsin solution, Basic Red 9, Magenta O, Basic Violet 14, 58969-01-0 (component) |
|---|---|
| Purity | for microscopy |
| Appearance | Dark red liquid |
| CAS-Number | 632-99-5 (component) |
| Molecular Formula | C20H20ClN3 |
| Molecular Weight | 337.85 |
| Identity | UV/Vis |
| Solvents | water |
| Refractive Index | n20/D 1.334 |
| Density | 0.999 g/ml at 20 °C |
| Smiles | NC1=CC=C(/C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C/3)=N)C=C1.Cl |
| InChi Key | NIKFYOSELWJIOF-SVFFXJIWSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | This product should not be used if colour has changed or if there are |
| Hazard statements | H350 |
| Precautionary statements | P201-P308 + P313 |
| GHS Symbol | GHS08 |
| Transportation | Not dangerous goods |
| References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) T.J. Beveridge, Biotech. Histochem. 76, 111 (2001), (3) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
| InChi | InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15,/h2-12,21H,22-23H2,1H3,1H/b20-14-,21-17?, |
| Quantity | 250 ml, 2.5 l, Bulk |
