Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Off-white to yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, chloroform
Documentation
- Safety Data Sheet (SDS)
- CDX C0103 MSDS.pdf
Available from stock
Green CMFDA
- SKU
- C0103
Categories: Fluorescent Detection, Reagents for Life Sciences
- Synonyms
- 5-Chloromethylfluorescein diacetate
- 136832-63-8
- CAS-Number
- C25H17ClO7
- Molecular Formula
- 464.85
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Hazard statements
- H315, H319, H335
- Precautionary statements
- P261, P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Thiol-reactive, cell permeant green fluorescent probe with high selectivity (Ex/Em: 492/517nm). This fluorescent dye is well suited for monitoring cell movement or location. Freely passes through cell membranes, where it is transformed into cell membrane-impermeant reaction products and is converted to the highly fluorescent probe (fluorescein) once the acetate groups are cleaved by intracellular esterases. The dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells and brightly fluorescent at physiological pH. The green excitation/emission spectra are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.
- Smiles
- CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(CCl)=C3)C1=CC=C(OC(C)=O)C=C1O2
- InChi Key
- IPJDHSYCSQAODE-UHFFFAOYSA-N
- InChi
- InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3
Thiol-reactive, cell permeant green fluorescent probe with high selectivity (Ex/Em: 492/517nm). This fluorescent dye is well suited for monitoring cell movement or location. Freely passes through cell membranes, where it is transformed into cell membrane-impermeant reaction products and is converted to the highly fluorescent probe (fluorescein) once the acetate groups are cleaved by intracellular esterases. The dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells and brightly fluorescent at physiological pH. The green excitation/emission spectra are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.
Additional information
| Synonyms | 5-Chloromethylfluorescein diacetate |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Off-white to yellow powder |
| CAS-Number | 136832-63-8 |
| Molecular Formula | C25H17ClO7 |
| Molecular Weight | 464.85 |
| Identity | 1H-NMR |
| Solvents | DMSO, chloroform |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(CCl)=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | IPJDHSYCSQAODE-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| InChi | InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3 |
| Quantity | 1 mg, 5 mg, 25 mg, Bulk |
